Detailed information of TA system TA093190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 216204..216733
• Replicon: chromosome
• Accession: NZ_CP026076
• Organism: Staphylococcus aureus strain FDAARGOS_4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: RK63_RS01400
• Protein ID: WP_000621175.1
• Coordinates: 216204..216566 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: RK63_RS01405
• Protein ID: WP_000948331.1
• Coordinates: 216563..216733 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RK63_RS01375	213182..213952	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
RK63_RS01380	213927..214406	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
RK63_RS01385	214408..214734	-	327	WP_001052491.1	anti-sigma factor antagonist	-
RK63_RS01390	214853..215854	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
RK63_RS01400	216204..216566	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RK63_RS01405	216563..216733	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
RK63_RS01410	216818..217966	-	1149	WP_001281145.1	alanine racemase	-
RK63_RS01415	218032..218391	-	360	WP_000581200.1	holo-ACP synthase	-
RK63_RS01420	218395..218886	-	492	WP_001205910.1	PH domain-containing protein	-
RK63_RS01425	218873..220456	-	1584	WP_039702418.1	PH domain-containing protein	-
RK63_RS01430	220449..220928	-	480	WP_001287088.1	hypothetical protein	-
RK63_RS01435	221136..221696	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T93190 WP_000621175.1 NZ_CP026076:c216566-216204 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T93190 NZ_CP026076:c216566-216204 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT93190 WP_000948331.1 NZ_CP026076:c216733-216563 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT93190 NZ_CP026076:c216733-216563 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1