Detailed information of TA system TA093132

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2331581..2332110
• Replicon: chromosome
• Accession: NZ_CP026072
• Organism: Staphylococcus aureus strain FDAARGOS_35

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: RK93_RS12080
• Protein ID: WP_000621175.1
• Coordinates: 2331748..2332110 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: RK93_RS12075
• Protein ID: WP_000948331.1
• Coordinates: 2331581..2331751 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RK93_RS12045	2326618..2327178	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
RK93_RS12050	2327386..2327865	+	480	WP_001287088.1	hypothetical protein	-
RK93_RS12055	2327858..2329441	+	1584	WP_001294626.1	PH domain-containing protein	-
RK93_RS12060	2329428..2329919	+	492	WP_001205910.1	PH domain-containing protein	-
RK93_RS12065	2329923..2330282	+	360	WP_000581200.1	holo-ACP synthase	-
RK93_RS12070	2330348..2331496	+	1149	WP_001281145.1	alanine racemase	-
RK93_RS12075	2331581..2331751	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
RK93_RS12080	2331748..2332110	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RK93_RS12090	2332460..2333466	+	1007	Protein_2270	PP2C family protein-serine/threonine phosphatase	-
RK93_RS12095	2333585..2333911	+	327	WP_001052491.1	anti-sigma factor antagonist	-
RK93_RS12100	2333913..2334392	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
RK93_RS12105	2334367..2335137	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T93132 WP_000621175.1 NZ_CP026072:2331748-2332110 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T93132 NZ_CP026072:2331748-2332110 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT93132 WP_000948331.1 NZ_CP026072:2331581-2331751 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT93132 NZ_CP026072:2331581-2331751 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1