Detailed information of TA system TA093073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2732455..2732984
• Replicon: chromosome
• Accession: NZ_CP026069
• Organism: Staphylococcus aureus strain FDAARGOS_25

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: RK83_RS14295
• Protein ID: WP_000621175.1
• Coordinates: 2732455..2732817 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: RK83_RS14300
• Protein ID: WP_000948331.1
• Coordinates: 2732814..2732984 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RK83_RS14270	2729434..2730204	-	771	WP_001041106.1	RNA polymerase sigma factor SigB	-
RK83_RS14275	2730179..2730658	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
RK83_RS14280	2730660..2730986	-	327	WP_001052491.1	anti-sigma factor antagonist	-
RK83_RS14285	2731105..2732106	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
RK83_RS14295	2732455..2732817	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RK83_RS14300	2732814..2732984	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
RK83_RS14305	2733069..2734217	-	1149	WP_001281154.1	alanine racemase	-
RK83_RS14310	2734283..2734642	-	360	WP_000581197.1	holo-ACP synthase	-
RK83_RS14315	2734646..2735137	-	492	WP_001205912.1	PH domain-containing protein	-
RK83_RS14320	2735124..2736707	-	1584	WP_001294626.1	PH domain-containing protein	-
RK83_RS14325	2736700..2737179	-	480	WP_001287087.1	hypothetical protein	-
RK83_RS14330	2737387..2737947	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T93073 WP_000621175.1 NZ_CP026069:c2732817-2732455 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T93073 NZ_CP026069:c2732817-2732455 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT93073 WP_000948331.1 NZ_CP026069:c2732984-2732814 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT93073 NZ_CP026069:c2732984-2732814 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1