Detailed information of TA system TA093003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 2352204..2352980
• Replicon: chromosome
• Accession: NZ_CP026064
• Organism: Staphylococcus aureus strain FDAARGOS_19

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: -
• Locus tag: RK77_RS12090
• Protein ID: WP_000031112.1
• Coordinates: 2352828..2352980 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: RK77_RS12085
• Protein ID: WP_001251224.1
• Coordinates: 2352204..2352803 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RK77_RS12065	2348121..2349578	+	1458	WP_000649910.1	ABC transporter permease subunit	-
RK77_RS12070	2349571..2350293	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
RK77_RS12075	2350443..2351570	+	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
RK77_RS12080	2351575..2352045	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
RK77_RS12085	2352204..2352803	+	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
RK77_RS12090	2352828..2352980	+	153	WP_000031112.1	hypothetical protein	Toxin
RK77_RS12095	2353815..2354210	+	396	WP_000901023.1	hypothetical protein	-
RK77_RS12100	2354406..2355791	+	1386	WP_000116232.1	class II fumarate hydratase	-
RK77_RS12105	2356253..2357074	-	822	WP_000669382.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5966.31 Da
• Isoelectric Point: 3.8962

>T93003 WP_000031112.1 NZ_CP026064:2352828-2352980 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELSKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T93003 NZ_CP026064:2352828-2352980 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAAGTAAAGAAATGGAACAAATTTCCGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT93003 WP_001251224.1 NZ_CP026064:2352204-2352803 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT93003 NZ_CP026064:2352204-2352803 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Antitoxin

• AlphaFold DB: W8U4V4