Detailed information of TA system TA092919

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4996489..4997109
• Replicon: chromosome
• Accession: NZ_CP026045
• Organism: Citrobacter freundii strain FDAARGOS_61

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: MC47_RS24360
• Protein ID: WP_002892050.1
• Coordinates: 4996891..4997109 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: MC47_RS24355
• Protein ID: WP_047413009.1
• Coordinates: 4996489..4996863 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MC47_RS24345	4991635..4992828	+	1194	WP_043015418.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
MC47_RS24350	4992851..4996000	+	3150	WP_043015417.1	multidrug efflux RND transporter permease subunit	-
MC47_RS24355	4996489..4996863	+	375	WP_047413009.1	Hha toxicity modulator TomB	Antitoxin
MC47_RS24360	4996891..4997109	+	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
MC47_RS24365	4997290..4997841	+	552	WP_043015416.1	maltose O-acetyltransferase	-
MC47_RS24370	4997958..4998428	+	471	WP_043015415.1	YlaC family protein	-
MC47_RS24375	4998508..4998648	-	141	WP_005125190.1	type B 50S ribosomal protein L36	-
MC47_RS24380	4998650..4998910	-	261	WP_043015414.1	type B 50S ribosomal protein L31	-
MC47_RS24385	4999099..5000652	+	1554	WP_047413013.1	EAL domain-containing protein	-
MC47_RS24390	5000704..5001057	-	354	WP_003831033.1	DUF1428 family protein	-
MC47_RS24395	5001122..5001751	-	630	WP_043015412.1	membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T92919 WP_002892050.1 NZ_CP026045:4996891-4997109 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T92919 NZ_CP026045:4996891-4997109 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACTGATTATTTGATGCGCCTGCGACGTTGTCAGACAATTGACACACTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCACCGCCTTGCTGAAT
TGACTATGAATAAGCTCTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14441.26 Da
• Isoelectric Point: 5.5716

>AT92919 WP_047413009.1 NZ_CP026045:4996489-4996863 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEENKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT92919 NZ_CP026045:4996489-4996863 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCCACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGAGAATAAGCTGATTGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGTCT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2