Detailed information of TA system TA092917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3673514..3674153
• Replicon: chromosome
• Accession: NZ_CP026045
• Organism: Citrobacter freundii strain FDAARGOS_61

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: MC47_RS17900
• Protein ID: WP_047411120.1
• Coordinates: 3673514..3673690 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: MC47_RS17905
• Protein ID: WP_123915356.1
• Coordinates: 3673737..3674153 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MC47_RS17880	3670292..3670522	-	231	WP_043016251.1	DUF2554 family protein	-
MC47_RS17885	3670712..3670837	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
MC47_RS17890	3670837..3671847	-	1011	WP_043016252.1	cytochrome d ubiquinol oxidase subunit II	-
MC47_RS17895	3671847..3673250	-	1404	WP_047411117.1	cytochrome ubiquinol oxidase subunit I	-
MC47_RS17900	3673514..3673690	+	177	WP_047411120.1	type II toxin-antitoxin system HicA family toxin	Toxin
MC47_RS17905	3673737..3674153	+	417	WP_123915356.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
MC47_RS17910	3674246..3675655	+	1410	WP_047411123.1	PLP-dependent aminotransferase family protein	-
MC47_RS17915	3675985..3677130	+	1146	WP_043016257.1	ABC transporter substrate-binding protein	-
MC47_RS17920	3677147..3678163	+	1017	WP_047411125.1	ABC transporter ATP-binding protein	-
MC47_RS17925	3678164..3679108	+	945	WP_043016259.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6784.77 Da
• Isoelectric Point: 11.2510

>T92917 WP_047411120.1 NZ_CP026045:3673514-3673690 [Citrobacter freundii]
VKQSEFRRWLESQGVEVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIQKQLGLQ

Nucleotide

• Length: 177 bp

>T92917 NZ_CP026045:3673514-3673690 [Citrobacter freundii]
GTGAAGCAAAGTGAGTTCAGACGCTGGCTTGAATCTCAGGGAGTTGAAGTTACAAACGGCTCAAATCATCTGAAGTTGAG
GTATCAGGGAAGGCGTAGCGTTATGCCCAGGCATCCTGGTGATGAGATAAAAGAGGCTCTGCGAAAAGCGATCCAAAAGC
AGCTAGGCTTACAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15002.27 Da
• Isoelectric Point: 4.5468

>AT92917 WP_123915356.1 NZ_CP026045:3673737-3674153 [Citrobacter freundii]
MRYPVNLVPAVEGGFVVSFPDIPEALTQGETRHDALQAAQAALVTAFEFYFDDNEPIPLPSAVVAGADYVEIPLSVASKV
LLLNAFLESNITQQELANRIGRPKQEITRLFDLKHATKIDAVQIAARALGKELSLAML

Nucleotide

• Length: 417 bp

>AT92917 NZ_CP026045:3673737-3674153 [Citrobacter freundii]
ATGCGTTATCCCGTTAATCTAGTACCCGCCGTGGAAGGTGGGTTTGTGGTGTCTTTTCCGGATATTCCTGAAGCATTAAC
TCAGGGAGAAACCCGTCATGATGCTTTGCAGGCTGCGCAAGCCGCATTGGTCACCGCTTTTGAATTCTATTTCGACGATA
ACGAACCGATTCCTTTGCCTTCGGCGGTTGTCGCTGGCGCTGACTATGTGGAAATACCACTGAGCGTTGCGTCTAAAGTT
CTGTTGTTAAATGCCTTCCTTGAATCGAATATTACCCAGCAGGAACTGGCAAACAGGATCGGCAGGCCTAAGCAGGAAAT
CACTCGCTTATTCGATTTAAAGCACGCGACAAAAATTGATGCTGTGCAGATAGCTGCCCGCGCCTTAGGCAAAGAGCTGT
CATTAGCGATGTTGTAA