Detailed information of TA system TA092902

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 398896..399667
• Replicon: chromosome
• Accession: NZ_CP026045
• Organism: Citrobacter freundii strain FDAARGOS_61

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: MC47_RS01920
• Protein ID: WP_047406475.1
• Coordinates: 398896..399285 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: MC47_RS01925
• Protein ID: WP_172626128.1
• Coordinates: 399341..399667 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MC47_RS01890	394141..394503	+	363	WP_047406455.1	endoribonuclease SymE	-
MC47_RS01895	394549..395184	-	636	WP_047406458.1	HNH endonuclease	-
MC47_RS01900	395325..396197	+	873	WP_047406461.1	HNH endonuclease	-
MC47_RS01905	396319..397122	-	804	WP_047406464.1	helix-turn-helix transcriptional regulator	-
MC47_RS01910	397500..397898	-	399	WP_047406467.1	hypothetical protein	-
MC47_RS01915	397967..398809	-	843	WP_047406472.1	DUF4942 domain-containing protein	-
MC47_RS01920	398896..399285	-	390	WP_047406475.1	TA system toxin CbtA family protein	Toxin
MC47_RS01925	399341..399667	-	327	WP_172626128.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MC47_RS01930	399725..399946	-	222	WP_047406482.1	DUF987 domain-containing protein	-
MC47_RS01935	399961..400440	-	480	WP_047406484.1	DNA repair protein RadC	-
MC47_RS01940	400452..400901	-	450	WP_047406487.1	antirestriction protein	-
MC47_RS01945	400934..401755	-	822	WP_047406489.1	DUF945 domain-containing protein	-
MC47_RS01950	402046..403032	-	987	WP_047406493.1	hypothetical protein	-
MC47_RS01955	403032..403214	-	183	WP_047406496.1	hypothetical protein	-
MC47_RS01960	403867..404478	+	612	WP_047413280.1	inovirus Gp2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	397500..417676	20176

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14545.68 Da
• Isoelectric Point: 10.2738

>T92902 WP_047406475.1 NZ_CP026045:c399285-398896 [Citrobacter freundii]
MQTKSSQLMREALSRPSAVGVWQTLLTFLLEHHYGLTLNDTPFSHEQVIQQHIDAGISLADALNFIVEKYELLRTDRPGF
SILTQSPFITPIDILRARKATGLMNRGTYKEVTAITRGQHPQARTSGKR

Nucleotide

• Length: 390 bp

>T92902 NZ_CP026045:c399285-398896 [Citrobacter freundii]
ATGCAAACCAAATCATCCCAACTCATGCGGGAGGCATTGTCACGTCCGTCTGCCGTGGGCGTCTGGCAGACCCTGCTGAC
CTTCCTGCTGGAACACCACTATGGTCTCACGCTTAATGACACGCCGTTCAGTCATGAGCAGGTGATTCAGCAGCATATCG
ACGCGGGTATCTCGCTGGCCGATGCCCTGAATTTTATTGTCGAAAAGTATGAACTGCTTCGCACTGACCGGCCAGGCTTC
AGCATCCTGACGCAGTCTCCGTTTATCACACCCATAGATATCCTCCGGGCCAGAAAGGCTACAGGGCTGATGAACCGAGG
CACGTATAAAGAGGTGACGGCTATTACCAGAGGACAACATCCGCAGGCCAGGACTTCAGGCAAGCGATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12023.74 Da
• Isoelectric Point: 6.6233

>AT92902 WP_172626128.1 NZ_CP026045:c399667-399341 [Citrobacter freundii]
ISAPWWGLRRSVSPCFGARLVQEGNRLHYLADRANFDGQFCDADLRHLDQAFPVLMKQLELMLTSGELNSLHQHCVTLYA
KGLTCEADTLGSHGYVYIAIYPTPAVTA

Nucleotide

• Length: 327 bp

>AT92902 NZ_CP026045:c399667-399341 [Citrobacter freundii]
ATCAGCGCCCCCTGGTGGGGGCTCAGACGCAGTGTCTCCCCGTGTTTTGGGGCACGTCTGGTCCAGGAGGGAAATCGCCT
GCATTACCTGGCTGACCGGGCAAACTTTGATGGTCAGTTCTGCGATGCGGATTTGCGCCATCTTGACCAGGCTTTCCCGG
TACTGATGAAACAGCTGGAGTTAATGCTCACCAGCGGTGAACTGAATTCCCTCCACCAGCACTGCGTCACGTTATATGCT
AAAGGGCTGACCTGTGAGGCCGATACGCTCGGTTCGCATGGTTACGTTTATATCGCGATTTATCCCACTCCTGCCGTCAC
GGCATAA