Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2567999..2568635 Replicon chromosome
Accession NZ_CP026039
Organism Bacillus subtilis strain PK1_2

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag C2I05_RS13585 Protein ID WP_003156187.1
Coordinates 2568285..2568635 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag C2I05_RS13580 Protein ID WP_003225183.1
Coordinates 2567999..2568280 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2I05_RS13560 (C2I05_13545) 2564358..2564957 - 600 WP_249848383.1 rhomboid family intramembrane serine protease -
C2I05_RS13565 (C2I05_13550) 2565052..2565417 + 366 WP_014475874.1 holo-ACP synthase -
C2I05_RS13570 (C2I05_13555) 2565583..2566599 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
C2I05_RS13575 (C2I05_13560) 2566714..2567883 + 1170 WP_015252766.1 alanine racemase -
C2I05_RS13580 (C2I05_13565) 2567999..2568280 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
C2I05_RS13585 (C2I05_13570) 2568285..2568635 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
C2I05_RS13590 (C2I05_13575) 2568751..2569575 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
C2I05_RS13595 (C2I05_13580) 2569580..2569945 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
C2I05_RS13600 (C2I05_13585) 2569949..2570350 + 402 WP_003225192.1 serine/threonine-protein kinase RsbT -
C2I05_RS13605 (C2I05_13590) 2570362..2571369 + 1008 WP_014478900.1 phosphoserine phosphatase RsbU -
C2I05_RS13610 (C2I05_13595) 2571438..2571767 + 330 WP_014475877.1 anti-sigma factor antagonist RsbV -
C2I05_RS13615 (C2I05_13600) 2571764..2572246 + 483 WP_021481702.1 anti-sigma B factor RsbW -
C2I05_RS13620 (C2I05_13605) 2572212..2573000 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
C2I05_RS13625 (C2I05_13610) 2573000..2573599 + 600 WP_120027794.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T92888 WP_003156187.1 NZ_CP026039:2568285-2568635 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T92888 NZ_CP026039:2568285-2568635 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT92888 WP_003225183.1 NZ_CP026039:2567999-2568280 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT92888 NZ_CP026039:2567999-2568280 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References