92789

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 262552..263231 Replicon chromosome
Accession NZ_CP026027
Organism Escherichia coli strain LIM

Toxin (Protein)


Gene name ykfI Uniprot ID P77692
Locus tag C2H82_RS01285 Protein ID WP_000854672.1
Coordinates 262552..262893 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID Q47684
Locus tag C2H82_RS01290 Protein ID WP_000070395.1
Coordinates 262914..263231 (-) Length 106 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2H82_RS01260 257829..258230 + 402 WP_000174689.1 sigma factor-binding protein Crl -
C2H82_RS01265 258269..259324 - 1056 WP_000749863.1 phosphoporin PhoE -
C2H82_RS01270 259612..260715 + 1104 WP_001285288.1 glutamate 5-kinase -
C2H82_RS01275 260727..261980 + 1254 WP_000893278.1 glutamate-5-semialdehyde dehydrogenase -
C2H82_RS01285 262552..262893 - 342 WP_000854672.1 type IV toxin-antitoxin system toxin YkfI Toxin
C2H82_RS01290 262914..263231 - 318 WP_000070395.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
C2H82_RS01295 263250..263471 - 222 WP_000691994.1 DUF987 domain-containing protein -
C2H82_RS01300 263480..263956 - 477 WP_000811693.1 RadC family protein -
C2H82_RS01305 263972..264430 - 459 WP_000211838.1 antirestriction protein -
C2H82_RS01310 264528..264767 - 240 WP_000194654.1 DUF905 domain-containing protein -
C2H82_RS01315 264844..265311 - 468 WP_001547765.1 hypothetical protein -
C2H82_RS01320 265334..265777 - 444 WP_000824223.1 hypothetical protein -
C2H82_RS01325 265777..266004 - 228 WP_001548158.1 hypothetical protein -
C2H82_RS01330 266000..266191 - 192 Protein_249 DeoR family transcriptional regulator -
C2H82_RS01335 266408..267229 - 822 WP_000197389.1 DUF945 domain-containing protein -
C2H82_RS01340 267321..268184 - 864 WP_001065553.1 GTPase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(6-104)


Sequences


Toxin        


Download         Length: 114 a.a.        Molecular weight: 12905.01 Da        Isoelectric Point: 9.6543

>T92789 WP_000854672.1 NZ_CP026027:c262893-262552 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T92789 NZ_CP026027:c262893-262552 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11939.59 Da        Isoelectric Point: 6.7391

>AT92789 WP_000070395.1 NZ_CP026027:c263231-262914 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT92789 NZ_CP026027:c263231-262914 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB P77692


Antitoxin

Source ID Structure
AlphaFold DB Q47684

References