Detailed information of TA system TA092750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 55925..56595
• Replicon: plasmid p11420-HVKP
• Accession: NZ_CP026024
• Organism: Klebsiella pneumoniae strain 11420

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: C2D59_RS26125
• Protein ID: WP_004213072.1
• Coordinates: 55925..56368 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: C2D59_RS26130
• Protein ID: WP_004213073.1
• Coordinates: 56365..56595 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2D59_RS26090	51337..51612	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
C2D59_RS26095	51675..52166	+	492	WP_011251327.1	DM13 domain-containing protein	-
C2D59_RS26100	52215..53135	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
C2D59_RS26105	53226..53632	+	407	Protein_57	GAF domain-containing protein	-
C2D59_RS28105	54147..54781	-	635	Protein_58	response regulator	-
C2D59_RS26115	55198..55491	+	294	Protein_59	transposase	-
C2D59_RS26120	55495..55911	+	417	Protein_60	restriction endonuclease	-
C2D59_RS26125	55925..56368	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
C2D59_RS26130	56365..56595	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C2D59_RS26135	57203..58336	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
C2D59_RS26140	58352..58645	+	294	WP_004213076.1	hypothetical protein	-
C2D59_RS26145	58635..58841	-	207	WP_004213077.1	hypothetical protein	-
C2D59_RS26150	59193..59483	+	291	WP_004213078.1	hypothetical protein	-
C2D59_RS26155	59473..60372	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA	1..229796	229796

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T92750 WP_004213072.1 NZ_CP026024:c56368-55925 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T92750 NZ_CP026024:c56368-55925 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT92750 WP_004213073.1 NZ_CP026024:c56595-56365 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT92750 NZ_CP026024:c56595-56365 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620