Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5360688..5361313 Replicon chromosome
Accession NZ_CP026023
Organism Klebsiella pneumoniae strain 11420

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag C2D59_RS25455 Protein ID WP_012737091.1
Coordinates 5360688..5361071 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag C2D59_RS25460 Protein ID WP_004150355.1
Coordinates 5361071..5361313 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2D59_RS25440 5358054..5358956 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
C2D59_RS25445 5358953..5359588 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
C2D59_RS25450 5359585..5360514 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
C2D59_RS25455 5360688..5361071 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
C2D59_RS25460 5361071..5361313 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
C2D59_RS25465 5361518..5362435 + 918 WP_012737090.1 alpha/beta hydrolase -
C2D59_RS25470 5362449..5363390 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
C2D59_RS25475 5363435..5363872 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
C2D59_RS25480 5363869..5364729 - 861 WP_004146232.1 virulence factor BrkB family protein -
C2D59_RS25485 5364723..5365322 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T92748 WP_012737091.1 NZ_CP026023:c5361071-5360688 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T92748 NZ_CP026023:c5361071-5360688 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT92748 WP_004150355.1 NZ_CP026023:c5361313-5361071 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT92748 NZ_CP026023:c5361313-5361071 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References