Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1469192..1469928 Replicon chromosome
Accession NZ_CP026017
Organism Klebsiella pneumoniae strain 13190

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag C2D61_RS07210 Protein ID WP_032433360.1
Coordinates 1469446..1469928 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag C2D61_RS07205 Protein ID WP_003026799.1
Coordinates 1469192..1469458 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2D61_RS07180 1464838..1465977 + 1140 WP_032433368.1 mannitol dehydrogenase -
C2D61_RS07185 1466006..1466668 + 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
C2D61_RS07190 1466652..1467656 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
C2D61_RS07195 1467674..1468306 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
C2D61_RS07200 1468316..1468879 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
C2D61_RS07205 1469192..1469458 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
C2D61_RS07210 1469446..1469928 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
C2D61_RS07215 1470289..1470888 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
C2D61_RS07220 1471101..1472045 - 945 WP_077254249.1 fimbrial protein -
C2D61_RS07225 1472057..1472635 - 579 WP_032432061.1 type 1 fimbrial protein -
C2D61_RS07230 1472639..1473379 - 741 WP_050597964.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1437137..1480871 43734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T92700 WP_032433360.1 NZ_CP026017:1469446-1469928 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T92700 NZ_CP026017:1469446-1469928 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT92700 WP_003026799.1 NZ_CP026017:1469192-1469458 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT92700 NZ_CP026017:1469192-1469458 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References