Detailed information of TA system TA092695

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 83240..83765
• Replicon: plasmid p13450-2
• Accession: NZ_CP026016
• Organism: Klebsiella variicola strain 13450

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: C2D62_RS29065
• Protein ID: WP_119312295.1
• Coordinates: 83460..83765 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: R4WC25
• Locus tag: C2D62_RS29060
• Protein ID: WP_015632547.1
• Coordinates: 83240..83458 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2D62_RS29040	78331..79496	+	1166	WP_100248964.1	IS3-like element ISKpn37 family transposase	-
C2D62_RS29045	80180..80530	-	351	WP_015632544.1	hypothetical protein	-
C2D62_RS29050	81109..81294	+	186	WP_032433930.1	hypothetical protein	-
C2D62_RS29055	81367..82386	-	1020	WP_138089330.1	hypothetical protein	-
C2D62_RS29060	83240..83458	+	219	WP_015632547.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
C2D62_RS29065	83460..83765	+	306	WP_119312295.1	type II toxin-antitoxin system toxin CcdB	Toxin
C2D62_RS29070	83952..84956	+	1005	WP_153242788.1	hypothetical protein	-
C2D62_RS29075	85154..85939	+	786	WP_153242790.1	site-specific integrase	-
C2D62_RS29080	86006..86164	-	159	WP_153242792.1	replication initiation protein	-
C2D62_RS29085	86229..86405	-	177	Protein_100	IS5/IS1182 family transposase	-
C2D62_RS29090	86813..87901	+	1089	WP_137963429.1	alpha/beta fold hydrolase	-
C2D62_RS29095	88129..88617	+	489	WP_137963430.1	M15 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..109205	109205
	-	flank	IS/Tn	-	-	88711..89292	581

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11618.35 Da
• Isoelectric Point: 6.4674

>T92695 WP_119312295.1 NZ_CP026016:83460-83765 [Klebsiella variicola]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRLMTTDMASVPASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T92695 NZ_CP026016:83460-83765 [Klebsiella variicola]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGGCGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAGCTCTATCCGGTGG
TGCACATCGGGGACGAGAGCTGGCGCCTGATGACCACGGACATGGCCAGCGTCCCGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8362.31 Da
• Isoelectric Point: 4.7247

>AT92695 WP_015632547.1 NZ_CP026016:83240-83458 [Klebsiella variicola]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIDMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT92695 NZ_CP026016:83240-83458 [Klebsiella variicola]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGATATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2L1KT86