Detailed information of TA system TA092693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 332613..333135
• Replicon: plasmid p13450-1
• Accession: NZ_CP026014
• Organism: Klebsiella variicola strain 13450

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A7H0EVH6
• Locus tag: C2D62_RS28170
• Protein ID: WP_014839901.1
• Coordinates: 332851..333135 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A7H0EVH5
• Locus tag: C2D62_RS28165
• Protein ID: WP_014839900.1
• Coordinates: 332613..332861 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2D62_RS28140	327791..328147	+	357	WP_017901071.1	hypothetical protein	-
C2D62_RS28145	328521..328919	+	399	WP_014839899.1	H-NS histone family protein	-
C2D62_RS28150	329056..329664	+	609	WP_024266306.1	DUF2913 family protein	-
C2D62_RS28155	330208..330840	+	633	WP_001567369.1	hypothetical protein	-
C2D62_RS28160	330869..332272	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
C2D62_RS28165	332613..332861	+	249	WP_014839900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C2D62_RS28170	332851..333135	+	285	WP_014839901.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2D62_RS28175	333326..333802	+	477	WP_042934526.1	hypothetical protein	-
C2D62_RS28180	333808..334485	+	678	WP_042934501.1	hypothetical protein	-
C2D62_RS28185	334502..335221	-	720	WP_042934502.1	cation:proton antiporter	-
C2D62_RS28190	335462..336064	-	603	WP_014839902.1	hypothetical protein	-
C2D62_RS28195	336061..336582	-	522	WP_048336588.1	hypothetical protein	-
C2D62_RS28200	336663..337607	-	945	WP_014839903.1	DsbA family protein	-
C2D62_RS28205	337670..337879	-	210	WP_125316032.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 / ARR-3 / aac(6')-Ib-cr / fosA3 / blaSHV-12 / sul2 / aph(3'')-Ib / aph(6)-Id / qnrS1 / blaIMP-4 / catA2 / aac(3)-IId	-	1..344479	344479
	-	flank	IS/Tn	-	-	330869..332272	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10866.46 Da
• Isoelectric Point: 10.5831

>T92693 WP_014839901.1 NZ_CP026014:332851-333135 [Klebsiella variicola]
MTYKLAFNESALKEWKKLGHTIREQFKKKLSERLQNPRVPAAQLHGGKDQYKIKLHGAGYRLVYSVNDEIVTVTVIGVGK
RNNDDIYNATRHRN

Nucleotide

• Length: 285 bp

>T92693 NZ_CP026014:332851-333135 [Klebsiella variicola]
ATGACCTATAAGTTAGCCTTCAATGAATCAGCTTTGAAAGAATGGAAAAAACTGGGTCATACTATTCGGGAGCAATTCAA
AAAGAAGCTTTCCGAACGGCTTCAGAACCCACGGGTGCCCGCAGCACAACTCCATGGAGGTAAAGACCAGTATAAAATAA
AGCTCCATGGCGCGGGTTACCGACTGGTTTACAGTGTCAACGATGAAATTGTGACGGTCACCGTAATTGGGGTGGGCAAA
CGCAACAATGATGATATCTACAACGCAACCCGACACAGAAATTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9071.29 Da
• Isoelectric Point: 3.8832

>AT92693 WP_014839900.1 NZ_CP026014:332613-332861 [Klebsiella variicola]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMEMLEDQELGRIADERIGEDRIEVNID
DL

Nucleotide

• Length: 249 bp

>AT92693 NZ_CP026014:332613-332861 [Klebsiella variicola]
ATGGCATTTCAGGTTTTAACCACCACAGCAGCCAGCATTACAGAACTTAAACGTGACCCTATGGGTACCTTCAATGCAGG
AGAAGGTGCACCGGTAGCAATCCTGAATCGCAACGAACCCGCTTTTTACTGTGTACCACCAGCCCTGTATGCTCAGCTGA
TGGAAATGCTGGAAGATCAAGAGTTGGGGCGTATTGCTGATGAACGAATCGGTGAGGACCGAATCGAGGTCAACATTGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: A0A7H0EVH6

Antitoxin

• AlphaFold DB: A0A7H0EVH5