Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4196143..4196762 Replicon chromosome
Accession NZ_CP026013
Organism Klebsiella variicola strain 13450

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag C2D62_RS20245 Protein ID WP_002892050.1
Coordinates 4196544..4196762 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag C2D62_RS20240 Protein ID WP_008805436.1
Coordinates 4196143..4196517 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2D62_RS20230 4191298..4192491 + 1194 WP_040973691.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C2D62_RS20235 4192514..4195660 + 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit -
C2D62_RS20240 4196143..4196517 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
C2D62_RS20245 4196544..4196762 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
C2D62_RS20250 4196921..4197487 + 567 WP_022066286.1 maltose O-acetyltransferase -
C2D62_RS29545 4197459..4197584 - 126 Protein_4015 hypothetical protein -
C2D62_RS20255 4197624..4198094 + 471 WP_008805434.1 YlaC family protein -
C2D62_RS20260 4198063..4199520 - 1458 WP_022066287.1 PLP-dependent aminotransferase family protein -
C2D62_RS20265 4199621..4200319 + 699 WP_022066288.1 GNAT family N-acetyltransferase -
C2D62_RS20270 4200316..4200456 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
C2D62_RS20275 4200456..4200719 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T92688 WP_002892050.1 NZ_CP026013:4196544-4196762 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T92688 NZ_CP026013:4196544-4196762 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT92688 WP_008805436.1 NZ_CP026013:4196143-4196517 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT92688 NZ_CP026013:4196143-4196517 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References