Detailed information of TA system TA092677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1866073..1866663
• Replicon: chromosome
• Accession: NZ_CP026013
• Organism: Klebsiella variicola strain 13450

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: C2D62_RS08990
• Protein ID: WP_012541133.1
• Coordinates: 1866331..1866663 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: C2D62_RS08985
• Protein ID: WP_016161431.1
• Coordinates: 1866073..1866330 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2D62_RS08965	1861681..1862256	+	576	WP_012541129.1	hypothetical protein	-
C2D62_RS08970	1862435..1863271	+	837	WP_008804155.1	alpha/beta hydrolase	-
C2D62_RS08975	1863478..1864449	+	972	WP_012541130.1	sensor domain-containing diguanylate cyclase	-
C2D62_RS08980	1864446..1865546	-	1101	WP_022065756.1	hypothetical protein	-
C2D62_RS08985	1866073..1866330	+	258	WP_016161431.1	hypothetical protein	Antitoxin
C2D62_RS08990	1866331..1866663	+	333	WP_012541133.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C2D62_RS08995	1866994..1868094	-	1101	WP_022065434.1	Retron-type reverse transcriptase	-
C2D62_RS09000	1868209..1870455	-	2247	WP_032730759.1	retron Eco8 family effector endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11819.67 Da
• Isoelectric Point: 10.1839

>T92677 WP_012541133.1 NZ_CP026013:1866331-1866663 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNVARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Nucleotide

• Length: 333 bp

>T92677 NZ_CP026013:1866331-1866663 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTCGTGGTTCCCGTGACCAGCGGCGGAAACGTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9450.94 Da
• Isoelectric Point: 4.4915

>AT92677 WP_016161431.1 NZ_CP026013:1866073-1866330 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT92677 NZ_CP026013:1866073-1866330 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA