Detailed information of TA system TA092674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 317536..318122
• Replicon: chromosome
• Accession: NZ_CP026013
• Organism: Klebsiella variicola strain 13450

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: C2D62_RS01470
• Protein ID: WP_012967118.1
• Coordinates: 317754..318122 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: C2D62_RS01465
• Protein ID: WP_004174006.1
• Coordinates: 317536..317757 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2D62_RS01445	313685..314611	+	927	WP_012540389.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
C2D62_RS01450	314608..315885	+	1278	WP_008806971.1	branched chain amino acid ABC transporter permease LivM	-
C2D62_RS01455	315882..316649	+	768	WP_008806972.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
C2D62_RS01460	316651..317364	+	714	WP_022066270.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
C2D62_RS01465	317536..317757	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C2D62_RS01470	317754..318122	+	369	WP_012967118.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
C2D62_RS01475	318414..319730	+	1317	WP_022066271.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
C2D62_RS01480	319837..320724	+	888	WP_012967120.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
C2D62_RS01485	320721..321566	+	846	WP_008806976.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
C2D62_RS01490	321568..322638	+	1071	WP_022066272.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	314608..323375	8767

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (26-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13584.98 Da
• Isoelectric Point: 8.6410

>T92674 WP_012967118.1 NZ_CP026013:317754-318122 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGMTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T92674 NZ_CP026013:317754-318122 [Klebsiella variicola]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGACCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTATGACAGACGTGT
GGCGACTCGCTGCGATGCACCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCCGACTTCGTCGGCATGACCGTCGAGGCTGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT92674 WP_004174006.1 NZ_CP026013:317536-317757 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT92674 NZ_CP026013:317536-317757 [Klebsiella variicola]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCGGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A5E5YJY7