Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5170704..5171329 Replicon chromosome
Accession NZ_CP026011
Organism Klebsiella pneumoniae strain K2044

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag C2D63_RS24510 Protein ID WP_012737091.1
Coordinates 5170704..5171087 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag C2D63_RS24515 Protein ID WP_004150355.1
Coordinates 5171087..5171329 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2D63_RS24495 5168070..5168972 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
C2D63_RS24500 5168969..5169604 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
C2D63_RS24505 5169601..5170530 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
C2D63_RS24510 5170704..5171087 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
C2D63_RS24515 5171087..5171329 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
C2D63_RS24520 5171534..5172451 + 918 WP_012737090.1 alpha/beta hydrolase -
C2D63_RS24525 5172465..5173406 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
C2D63_RS24530 5173451..5173888 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
C2D63_RS24535 5173885..5174745 - 861 WP_004146232.1 virulence factor BrkB family protein -
C2D63_RS24540 5174739..5175338 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T92669 WP_012737091.1 NZ_CP026011:c5171087-5170704 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T92669 NZ_CP026011:c5171087-5170704 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT92669 WP_004150355.1 NZ_CP026011:c5171329-5171087 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT92669 NZ_CP026011:c5171329-5171087 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References