Detailed information of TA system TA092656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 1780924..1781567
• Replicon: chromosome
• Accession: NZ_CP026011
• Organism: Klebsiella pneumoniae strain K2044

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A4GZE5
• Locus tag: C2D63_RS08480
• Protein ID: WP_015874977.1
• Coordinates: 1780924..1781340 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A4GZE4
• Locus tag: C2D63_RS08485
• Protein ID: WP_015874976.1
• Coordinates: 1781337..1781567 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2D63_RS08465	1776327..1777442	-	1116	WP_015874980.1	salmochelin biosynthesis C-glycosyltransferase IroB	-
C2D63_RS08470	1778314..1780488	+	2175	WP_015874979.1	siderophore salmochelin receptor IroN	-
C2D63_RS08475	1780643..1780888	-	246	WP_032447699.1	hypothetical protein	-
C2D63_RS08480	1780924..1781340	-	417	WP_015874977.1	type II toxin-antitoxin system VapC family toxin	Toxin
C2D63_RS08485	1781337..1781567	-	231	WP_015874976.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C2D63_RS08490	1782010..1782390	+	381	WP_015874975.1	YrzE family protein	-
C2D63_RS08495	1782527..1783480	+	954	Protein_1677	IS21 family transposase	-
C2D63_RS08500	1783545..1784301	+	757	Protein_1678	AAA family ATPase	-
C2D63_RS08505	1784345..1784458	-	114	WP_015874970.1	hypothetical protein	-
C2D63_RS08510	1784701..1784910	-	210	WP_032447696.1	TraR/DksA family transcriptional regulator	-
C2D63_RS08515	1784900..1785481	-	582	WP_000937857.1	DUF2857 domain-containing protein	-
C2D63_RS08520	1785466..1785648	-	183	WP_072001695.1	hypothetical protein	-
C2D63_RS08525	1785670..1785855	-	186	WP_000205185.1	AlpA family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	rmpA / iroD / iroC / iroB / iroN	1742654..1786921	44267

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14945.39 Da
• Isoelectric Point: 8.5464

>T92656 WP_015874977.1 NZ_CP026011:c1781340-1780924 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAGLRGDRIVVSAVTYAEMRFGATGPKASPHHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T92656 NZ_CP026011:c1781340-1780924 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGGGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCACCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTACCGGGCCTGGTGCTGG
AAGACTGGGTAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8749.80 Da
• Isoelectric Point: 4.3533

>AT92656 WP_015874976.1 NZ_CP026011:c1781567-1781337 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMVEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT92656 NZ_CP026011:c1781567-1781337 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGTGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A4GZE5

Antitoxin

• AlphaFold DB: A4GZE4