Detailed information of TA system TA092650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 2155102..2155817
• Replicon: chromosome
• Accession: NZ_CP026009
• Organism: Actinobacillus pleuropneumoniae strain App6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CYK57_RS10215
• Protein ID: WP_005619039.1
• Coordinates: 2155102..2155530 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BT01
• Locus tag: CYK57_RS10220
• Protein ID: WP_005599447.1
• Coordinates: 2155530..2155817 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYK57_RS10200	2150687..2152393	-	1707	WP_005609259.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-
CYK57_RS10205	2152489..2152770	-	282	WP_005599441.1	DNA-directed RNA polymerase subunit omega	-
CYK57_RS10210	2152897..2154978	-	2082	WP_009875323.1	ATP-dependent DNA helicase RecG	-
CYK57_RS10215	2155102..2155530	-	429	WP_005619039.1	type II toxin-antitoxin system VapC family toxin	Toxin
CYK57_RS10220	2155530..2155817	-	288	WP_005599447.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CYK57_RS10225	2156023..2157738	+	1716	WP_009875324.1	proline--tRNA ligase	-
CYK57_RS10230	2157804..2157992	-	189	WP_005602684.1	hypothetical protein	-
CYK57_RS10235	2157995..2158576	-	582	WP_207618149.1	sigma-70 family RNA polymerase sigma factor	-
CYK57_RS10240	2158665..2159621	+	957	WP_005619040.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
CYK57_RS10245	2159621..2160214	+	594	WP_005599453.1	sulfoxide reductase heme-binding subunit YedZ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 16508.97 Da
• Isoelectric Point: 8.9724

>T92650 WP_005619039.1 NZ_CP026009:c2155530-2155102 [Actinobacillus pleuropneumoniae]
MFQYLFDTNIISELYKLGSNRMDTNVRQWLATIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQA
KSFSINNEIALLASEYHIPNKMDLNDAYIAATAKYHNLVLVTRNLKDFNRCDIRLFNPFEPN

Nucleotide

• Length: 429 bp

>T92650 NZ_CP026009:c2155530-2155102 [Actinobacillus pleuropneumoniae]
ATGTTTCAATATCTTTTTGATACCAACATCATTTCCGAGCTTTATAAGCTAGGAAGCAATAGAATGGATACCAATGTACG
ACAATGGTTAGCAACGATTAAACCGAGTCAGACGAATATTAGCTGTATCACGCTTTCCGAAATAAAAACGGGGATTTTAC
TCAAAGCTCGTAAAGATCCTATTCAAGCCGAGCGGTTAAATCATTGGTTTACCCATAATGTATTAAGCGTTTATCAAGCT
AAATCCTTTTCTATAAATAATGAAATTGCGTTACTGGCTTCTGAATACCACATTCCAAATAAAATGGATTTAAACGATGC
TTATATTGCCGCAACCGCTAAATATCATAATTTAGTGCTTGTTACTCGTAATTTGAAAGACTTCAACCGTTGCGATATAC
GTTTATTTAATCCTTTCGAACCGAATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11040.29 Da
• Isoelectric Point: 4.5578

>AT92650 WP_005599447.1 NZ_CP026009:c2155817-2155530 [Actinobacillus pleuropneumoniae]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE

Nucleotide

• Length: 288 bp

>AT92650 NZ_CP026009:c2155817-2155530 [Actinobacillus pleuropneumoniae]
ATGCCAATTACTATTACCTCTCGAGAATTTAATCAACACGCCAGTAAAGTGCTGAAAATGGCAGAAAAAGAACCGGTTTT
TATCACTAAATGGGGAAAAATTGTCAGCGTTGTCTCCAATTATGTTGATTACAAACAGCACACAACGGTTGAGCCTAGCT
TTGAAGAACTATTTGCCTCATCTACGGATGAATCGGTTAGCGATGAGTTTTGGCAAGATTTTGAACAGACATTAACAAAA
ATTCGCAAAAGTAATAAATCCGAACAAATAGATTTTGGAGATGAATAA

Structures

Antitoxin

• AlphaFold DB: A3N3C5