Detailed information of TA system TA092649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2071215..2071843
• Replicon: chromosome
• Accession: NZ_CP026009
• Organism: Actinobacillus pleuropneumoniae strain App6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CYK57_RS09695
• Protein ID: WP_009875032.1
• Coordinates: 2071445..2071843 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B3GYV7
• Locus tag: CYK57_RS09690
• Protein ID: WP_005613300.1
• Coordinates: 2071215..2071445 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYK57_RS09685	2069062..2070693	+	1632	WP_042655123.1	hypothetical protein	-
CYK57_RS09690	2071215..2071445	+	231	WP_005613300.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CYK57_RS09695	2071445..2071843	+	399	WP_009875032.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CYK57_RS09700	2071978..2072394	-	417	WP_005618204.1	DUF417 family protein	-
CYK57_RS09705	2072640..2073290	+	651	WP_005618207.1	DUF2202 domain-containing protein	-
CYK57_RS09710	2073470..2074249	-	780	WP_005602546.1	hypothetical protein	-
CYK57_RS09715	2074322..2075644	-	1323	WP_005620148.1	HslU--HslV peptidase ATPase subunit	-
CYK57_RS09720	2075699..2076220	-	522	WP_005618211.1	ATP-dependent protease subunit HslV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2055701..2074249	18548

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (6-53)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15105.39 Da
• Isoelectric Point: 7.4195

>T92649 WP_009875032.1 NZ_CP026009:2071445-2071843 [Actinobacillus pleuropneumoniae]
MLTYMLDTNIAIYVIKRRPIEVLDKFNLNSTRLCISSITAAELYYGAEKSQFPERNMAVIEDFLSRLTILDYTHKAATHF
GNIKAHLSKQGKIIGENDIHIAAHARSEGLVLVTNNLREFERVEGLRLDNWV

Nucleotide

• Length: 399 bp

>T92649 NZ_CP026009:2071445-2071843 [Actinobacillus pleuropneumoniae]
ATGCTAACTTATATGCTTGATACTAACATTGCGATTTATGTGATTAAACGCAGACCTATTGAAGTGCTGGATAAGTTTAA
TTTAAATTCTACCCGTCTTTGTATAAGTAGTATCACTGCAGCGGAGCTGTATTATGGTGCAGAGAAAAGCCAGTTTCCGG
AACGAAATATGGCAGTAATTGAGGATTTTCTTTCTCGTTTAACGATTCTTGATTATACGCATAAGGCGGCTACTCACTTT
GGCAATATCAAAGCACATTTATCTAAGCAAGGTAAGATCATTGGCGAGAACGATATTCATATTGCCGCACATGCAAGAAG
TGAAGGACTCGTATTAGTAACCAATAACTTGCGTGAATTTGAACGTGTGGAAGGATTGCGATTAGATAATTGGGTATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8625.71 Da
• Isoelectric Point: 4.5358

>AT92649 WP_005613300.1 NZ_CP026009:2071215-2071445 [Actinobacillus pleuropneumoniae]
MEASVFMTNRSQAVRLPAEVRFASDVKKLSVRVVGNERILAPINQSWDSFFLSSATVSDDFMTEREISTQAEREEL

Nucleotide

• Length: 231 bp

>AT92649 NZ_CP026009:2071215-2071445 [Actinobacillus pleuropneumoniae]
ATGGAAGCATCAGTATTTATGACAAACCGTAGTCAGGCGGTACGATTACCTGCAGAAGTGCGTTTTGCAAGTGATGTAAA
AAAACTTTCTGTAAGAGTGGTAGGCAATGAACGTATTTTGGCTCCAATTAACCAAAGTTGGGATAGCTTCTTTCTGAGTT
CGGCAACGGTAAGCGATGATTTCATGACAGAGCGTGAAATCAGCACTCAAGCCGAACGTGAGGAACTATAA

Structures

Antitoxin

• AlphaFold DB: A3N328