Detailed information of TA system TA092647

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1856401..1857132
• Replicon: chromosome
• Accession: NZ_CP026009
• Organism: Actinobacillus pleuropneumoniae strain App6

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A3N2I6
• Locus tag: CYK57_RS08635
• Protein ID: WP_009875245.1
• Coordinates: 1856401..1856868 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: B0BRD3
• Locus tag: CYK57_RS08640
• Protein ID: WP_005619269.1
• Coordinates: 1856872..1857132 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYK57_RS08610	1851759..1852130	-	372	WP_005598830.1	CrcB family protein	-
CYK57_RS08615	1852133..1853197	-	1065	WP_011848610.1	MFS transporter	-
CYK57_RS08620	1853292..1854401	+	1110	WP_009875243.1	anhydro-N-acetylmuramic acid kinase	-
CYK57_RS08625	1854455..1855369	+	915	WP_009875244.1	N-acetylmuramic acid 6-phosphate etherase	-
CYK57_RS08630	1855436..1856317	-	882	WP_005608878.1	50S ribosomal protein L11 methyltransferase	-
CYK57_RS08635	1856401..1856868	-	468	WP_009875245.1	GNAT family N-acetyltransferase	Toxin
CYK57_RS08640	1856872..1857132	-	261	WP_005619269.1	DUF1778 domain-containing protein	Antitoxin
CYK57_RS08645	1857253..1858713	-	1461	WP_009875246.1	metalloprotease TldD	-
CYK57_RS08650	1858843..1860102	+	1260	WP_005608887.1	tRNA lysidine(34) synthetase TilS	-
CYK57_RS08655	1860129..1860419	-	291	Protein_1676	DUF5389 family protein	-
CYK57_RS08660	1860421..1861314	-	894	WP_005598850.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (40-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17227.20 Da
• Isoelectric Point: 9.5125

>T92647 WP_009875245.1 NZ_CP026009:c1856868-1856401 [Actinobacillus pleuropneumoniae]
MHAPELLSEQHIVRYFQCGEVVLDQWLQRTALKNQQNNASKTFVVRDENKQVMGFYCLSAGSVSHQFVAGALRRNMPDPI
PVIILGRLAVDCSAQGKQLGVSMLKDAVLKSKTVANQIGVKALLVHALNPQAKQFYLKYGFSCSPIDEIVLMLKL

Nucleotide

• Length: 468 bp

>T92647 NZ_CP026009:c1856868-1856401 [Actinobacillus pleuropneumoniae]
ATGCACGCACCGGAACTGCTTTCCGAGCAACATATTGTGCGATATTTTCAATGTGGTGAGGTCGTGCTAGACCAATGGCT
ACAACGTACTGCATTGAAAAATCAGCAAAATAATGCCAGTAAAACCTTTGTTGTGCGTGATGAGAATAAACAGGTGATGG
GATTTTATTGTTTATCTGCCGGTTCGGTGAGCCATCAGTTTGTTGCAGGCGCATTAAGACGTAATATGCCCGATCCGATT
CCCGTGATTATTTTAGGTAGATTAGCGGTCGATTGCTCAGCACAGGGTAAGCAGTTAGGCGTATCAATGTTAAAAGATGC
GGTCTTAAAATCGAAGACGGTGGCGAATCAAATCGGGGTAAAAGCATTGCTTGTTCATGCGTTAAATCCGCAAGCAAAAC
AATTTTATTTAAAGTACGGTTTTTCATGTTCACCCATTGATGAGATAGTTTTAATGTTAAAACTCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9796.14 Da
• Isoelectric Point: 4.4883

>AT92647 WP_005619269.1 NZ_CP026009:c1857132-1856872 [Actinobacillus pleuropneumoniae]
MRTAPINLRAMPDQRDLIDYAASILGKTRSDFMLETACQAAQNVILDRTVFQLNEQAFEQFNRLLEQPTADNPGLDKLLN
TKAVWE

Nucleotide

• Length: 261 bp

>AT92647 NZ_CP026009:c1857132-1856872 [Actinobacillus pleuropneumoniae]
ATGAGAACTGCGCCAATTAATTTACGAGCGATGCCTGATCAGCGTGATTTAATTGATTATGCGGCAAGCATTTTAGGTAA
AACACGTTCTGACTTTATGTTAGAAACCGCCTGCCAAGCTGCCCAAAATGTGATTTTAGATCGTACCGTTTTCCAATTAA
ATGAGCAGGCTTTCGAGCAATTTAACCGATTGCTAGAACAGCCAACAGCAGATAATCCGGGGTTAGATAAACTGTTAAAT
ACCAAAGCGGTGTGGGAGTAG

Structures

Toxin

• AlphaFold DB: A3N2I6

Antitoxin

• AlphaFold DB: A3N2I7