Detailed information of TA system TA092645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 1572326..1572834
• Replicon: chromosome
• Accession: NZ_CP026009
• Organism: Actinobacillus pleuropneumoniae strain App6

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CYK57_RS07195
• Protein ID: WP_009875288.1
• Coordinates: 1572574..1572834 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A3N1S6
• Locus tag: CYK57_RS07190
• Protein ID: WP_005605062.1
• Coordinates: 1572326..1572577 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYK57_RS07150	1567862..1568410	-	549	WP_011848557.1	type IV pilus biogenesis/stability protein PilW	-
CYK57_RS07155	1568464..1569645	-	1182	WP_009875290.1	bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN	-
CYK57_RS07180	1570549..1571988	+	1440	WP_009875289.1	glutamate--tRNA ligase	-
CYK57_RS07190	1572326..1572577	+	252	WP_005605062.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
CYK57_RS07195	1572574..1572834	+	261	WP_009875288.1	Txe/YoeB family addiction module toxin	Toxin
CYK57_RS07200	1572991..1573158	-	168	WP_009875287.1	Trm112 family protein	-
CYK57_RS07205	1573160..1574140	-	981	WP_005608502.1	tetraacyldisaccharide 4'-kinase	-
CYK57_RS07210	1574234..1575493	-	1260	WP_005598324.1	ATP-dependent protease ATP-binding subunit ClpX	-
CYK57_RS07215	1575493..1576083	-	591	WP_005598326.1	ATP-dependent Clp endopeptidase proteolytic subunit ClpP	-
CYK57_RS07220	1576205..1576597	+	393	WP_005620423.1	SufE family protein	-
CYK57_RS07235	1576956..1577729	-	774	WP_009875285.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10452.00 Da
• Isoelectric Point: 8.0109

>T92645 WP_009875288.1 NZ_CP026009:1572574-1572834 [Actinobacillus pleuropneumoniae]
MKLTFSSNAWEDYLYWQKTDKIILKRINSLIKDIQRQPFEGIGKPEPLKFNLSGFWSRRINEEHRLIYTVEDETILIVAC
RYHYDQ

Nucleotide

• Length: 261 bp

>T92645 NZ_CP026009:1572574-1572834 [Actinobacillus pleuropneumoniae]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGTCTGATTAAAGATATTCAACGCCAACCATTCGAAGGCATAGGAAAGCCTGAACCGCTAAAATTCAATCTTTCGG
GCTTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACACCGTGGAAGATGAAACAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9320.62 Da
• Isoelectric Point: 4.5659

>AT92645 WP_005605062.1 NZ_CP026009:1572326-1572577 [Actinobacillus pleuropneumoniae]
MQAITYTEARQNLAGTMSKVAEDFEPILITRSKGGNCVLMSYEQYSSLEETAYLMRSPANAQRLLDSMAELKQGKGIERE
LIE

Nucleotide

• Length: 252 bp

>AT92645 NZ_CP026009:1572326-1572577 [Actinobacillus pleuropneumoniae]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACAATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATAGTTCACTTGAAGAAACCGCCTATT
TAATGCGTTCCCCGGCTAACGCTCAAAGATTACTAGACAGCATGGCAGAATTGAAGCAAGGGAAAGGCATTGAAAGAGAG
CTAATCGAATGA

Structures

Antitoxin

• AlphaFold DB: A3N1S6