Detailed information of TA system TA092644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 864779..865429
• Replicon: chromosome
• Accession: NZ_CP026009
• Organism: Actinobacillus pleuropneumoniae strain App6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B0BNU3
• Locus tag: CYK57_RS04010
• Protein ID: WP_005614926.1
• Coordinates: 864779..865168 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BNU4
• Locus tag: CYK57_RS04015
• Protein ID: WP_005604117.1
• Coordinates: 865169..865429 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYK57_RS03995	860356..861546	+	1191	WP_207618221.1	aromatic amino acid transaminase	-
CYK57_RS04000	861725..863011	-	1287	WP_009874699.1	Xaa-Pro aminopeptidase	-
CYK57_RS04005	863420..864643	-	1224	WP_009874700.1	ATP-binding protein	-
CYK57_RS04010	864779..865168	-	390	WP_005614926.1	type II toxin-antitoxin system VapC family toxin	Toxin
CYK57_RS04015	865169..865429	-	261	WP_005604117.1	hypothetical protein	Antitoxin
CYK57_RS04020	865563..867584	-	2022	WP_009874701.1	excinuclease ABC subunit B	-
CYK57_RS04025	867761..868573	+	813	WP_005607533.1	EfeM/EfeO family lipoprotein	-
CYK57_RS04030	868585..869778	+	1194	WP_009874702.1	deferrochelatase/peroxidase EfeB	-
CYK57_RS04035	869789..870409	+	621	WP_009874703.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14837.17 Da
• Isoelectric Point: 5.1295

>T92644 WP_005614926.1 NZ_CP026009:c865168-864779 [Actinobacillus pleuropneumoniae]
MYMLDTNTVSYFFRQDPTVVKKLQQLNPELICISSVTAAELFYGVKKRNNQKLTAFLNTFLSAITVMDWDYQVAEVYGQL
RAEMEREGKIMGVQDQMIGAHALATECVLVSSDKAFQFIPNLVLENWWK

Nucleotide

• Length: 390 bp

>T92644 NZ_CP026009:c865168-864779 [Actinobacillus pleuropneumoniae]
ATGTATATGCTAGATACTAATACGGTGAGTTATTTCTTTAGGCAAGATCCTACTGTGGTTAAGAAACTACAACAACTTAA
TCCGGAATTGATTTGTATTTCATCGGTCACGGCTGCAGAGTTGTTTTATGGCGTAAAAAAACGTAATAATCAGAAACTTA
CTGCATTTTTAAATACTTTTTTATCTGCGATTACGGTGATGGATTGGGATTATCAAGTGGCGGAAGTTTATGGTCAGCTG
CGAGCAGAGATGGAACGAGAAGGCAAAATCATGGGCGTTCAAGATCAAATGATTGGAGCTCATGCCCTAGCAACAGAATG
TGTTCTTGTTTCCAGCGATAAAGCCTTTCAATTTATCCCTAATCTCGTATTAGAAAATTGGTGGAAGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10289.63 Da
• Isoelectric Point: 5.1867

>AT92644 WP_005604117.1 NZ_CP026009:c865429-865169 [Actinobacillus pleuropneumoniae]
MEKVARLFRNGRNQAVRLPVEFEFNAKQVYVFKEANGDITLSTRSRNQQNWQKVFELLPMLECDEQFLSEQERKQEMATR
DPFEGF

Nucleotide

• Length: 261 bp

>AT92644 NZ_CP026009:c865429-865169 [Actinobacillus pleuropneumoniae]
ATGGAAAAGGTCGCACGTTTATTTAGAAACGGACGTAATCAAGCCGTACGATTACCGGTTGAATTTGAGTTTAATGCGAA
GCAAGTTTATGTGTTTAAAGAAGCAAACGGCGATATTACGCTTTCGACACGTTCTCGCAATCAGCAAAATTGGCAGAAAG
TCTTTGAGTTATTACCAATGCTTGAATGTGATGAACAGTTTTTATCGGAACAAGAACGCAAGCAAGAAATGGCAACTCGA
GATCCGTTCGAGGGCTTTTAA

Structures

Toxin

• AlphaFold DB: A3N029

Antitoxin

• AlphaFold DB: A3N030