92641

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 457284..457843 Replicon chromosome
Accession NZ_CP026005
Organism Brucella melitensis strain CIIMS-PH-3

Toxin (Protein)


Gene name brnT Uniprot ID A0A7U8PD14
Locus tag C0R52_RS02305 Protein ID WP_002966807.1
Coordinates 457284..457529 (+) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID C0RIV1
Locus tag C0R52_RS02310 Protein ID WP_004683740.1
Coordinates 457529..457843 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C0R52_RS02285 453128..454579 - 1452 WP_006135932.1 hypothetical protein -
C0R52_RS02290 454991..455683 - 693 WP_006135935.1 hypothetical protein -
C0R52_RS02300 456513..457151 - 639 Protein_428 tRNA-guanine transglycosylase -
C0R52_RS02305 457284..457529 + 246 WP_002966807.1 BrnT family toxin Toxin
C0R52_RS02310 457529..457843 + 315 WP_004683740.1 BrnA antitoxin family protein Antitoxin
C0R52_RS02320 458191..458361 + 171 WP_002964095.1 DUF1508 domain-containing protein -
C0R52_RS02325 458709..459419 - 711 WP_004683739.1 porin family protein -
C0R52_RS02330 459765..460241 - 477 WP_002966806.1 hypothetical protein -
C0R52_RS02335 460264..460539 - 276 WP_002964092.1 hypothetical protein -
C0R52_RS02340 460536..460766 - 231 WP_002964091.1 hypothetical protein -
C0R52_RS02345 460763..461467 - 705 WP_102207964.1 hypothetical protein -
C0R52_RS02350 461517..461720 + 204 WP_002964088.1 hypothetical protein -
C0R52_RS02355 461717..461932 + 216 WP_005968947.1 hypothetical protein -
C0R52_RS02360 461935..462138 + 204 WP_004683737.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - wbdA / wboA 450014..463910 13896
- flank IS/Tn - - 455799..456443 644


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9162.82 Da        Isoelectric Point: 10.3158

>T92641 WP_002966807.1 NZ_CP026005:457284-457529 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T92641 NZ_CP026005:457284-457529 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11653.48 Da        Isoelectric Point: 10.5824

>AT92641 WP_004683740.1 NZ_CP026005:457529-457843 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT92641 NZ_CP026005:457529-457843 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8PD14

Antitoxin

Source ID Structure
AlphaFold DB C0RIV1

References