Detailed information of TA system TA092633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 282416..283026
• Replicon: plasmid p-unname2
• Accession: NZ_CP025986
• Organism: Ralstonia solanacearum strain RSCM

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A454TTM1
• Locus tag: CYD94_RS18945
• Protein ID: WP_058907722.1
• Coordinates: 282718..283026 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CYD94_RS18940
• Protein ID: WP_016722339.1
• Coordinates: 282416..282721 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYD94_RS18910	277914..278399	+	486	WP_016726511.1	hypothetical protein	-
CYD94_RS18915	278730..279011	-	282	WP_058907724.1	hypothetical protein	-
CYD94_RS18920	279143..279427	-	285	WP_011003391.1	hypothetical protein	-
CYD94_RS18925	279732..279949	+	218	Protein_219	transposase	-
CYD94_RS18930	280116..280784	+	669	WP_058907723.1	hypothetical protein	-
CYD94_RS18935	280890..282203	-	1314	WP_029240574.1	metabolite/H+ symporter	-
CYD94_RS18940	282416..282721	-	306	WP_016722339.1	putative addiction module antidote protein	Antitoxin
CYD94_RS18945	282718..283026	-	309	WP_058907722.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CYD94_RS18950	283102..284301	-	1200	WP_058907721.1	MFS transporter	-
CYD94_RS18955	284428..285249	-	822	WP_011003398.1	GntR family transcriptional regulator	-
CYD94_RS18960	285427..285978	-	552	WP_011003399.1	helix-turn-helix transcriptional regulator	-
CYD94_RS18965	286111..286710	+	600	WP_071507364.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	flgC / flgG / fliM / fliG / fliI / icl / htpB / acrB / adeG / cheY / cheB / cheW / motA / cyaB	1..2212170	2212170

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-88)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11863.66 Da
• Isoelectric Point: 7.8930

>T92633 WP_058907722.1 NZ_CP025986:c283026-282718 [Ralstonia solanacearum]
MLTIRTTEIFDDWFCSLRDKAAQRRIQVRIDRLQMGHPGNMKAVRDGIRELRIDHGPGDRLYFVRHGVVLIVLLCGGDKS
TQETDIRRAIDLSRRLDVDDME

Nucleotide

• Length: 309 bp

>T92633 NZ_CP025986:c283026-282718 [Ralstonia solanacearum]
ATGCTGACGATCCGCACGACCGAAATCTTCGACGACTGGTTTTGCAGCCTCCGCGACAAGGCTGCGCAACGCCGCATCCA
GGTGCGCATCGACCGCCTGCAGATGGGCCACCCAGGCAACATGAAAGCGGTCCGTGACGGCATCCGTGAGCTGCGGATCG
ATCATGGGCCGGGCGACCGCTTGTACTTCGTGCGGCATGGCGTGGTCCTGATCGTACTGCTCTGCGGCGGCGACAAGTCG
ACGCAGGAAACCGATATCCGTCGCGCAATTGACCTGTCCCGCCGTCTCGACGTGGACGACATGGAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10967.57 Da
• Isoelectric Point: 7.2780

>AT92633 WP_016722339.1 NZ_CP025986:c282721-282416 [Ralstonia solanacearum]
MTMAQRTIKTRPWDSAEHLRTEADIAAYLDACLEEAGDDPAFITHALGVVARSRGMTQLARETGMTREGLYKALSEGGNP
SFATVLKVIRALGIRLHAVPT

Nucleotide

• Length: 306 bp

>AT92633 NZ_CP025986:c282721-282416 [Ralstonia solanacearum]
ATGACGATGGCCCAACGCACCATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTCCGGACCGAAGCGGACATCGCCGC
CTACCTCGACGCCTGCCTGGAAGAAGCCGGAGATGACCCCGCCTTCATCACGCATGCGCTGGGCGTGGTCGCGCGCTCGC
GCGGCATGACGCAGCTCGCCCGCGAGACAGGGATGACCCGCGAAGGGCTGTACAAGGCGCTTTCCGAGGGCGGCAATCCG
AGCTTTGCGACCGTGCTGAAGGTCATCCGGGCGCTCGGCATCCGGCTGCACGCCGTGCCGACCTGA

Structures

Toxin

• AlphaFold DB: A0A454TTM1