Detailed information of TA system TA092628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4220..4830
• Replicon: plasmid pEM76
• Accession: NZ_CP025981
• Organism: Escherichia marmotae strain HT073016

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: C1192_RS24630
• Protein ID: WP_038355952.1
• Coordinates: 4441..4830 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: C1192_RS24625
• Protein ID: WP_038355953.1
• Coordinates: 4220..4444 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1192_RS24605	1..978	+	978	WP_000361612.1	RepB family plasmid replication initiator protein	-
C1192_RS24610	1251..1991	-	741	WP_038355955.1	tyrosine-type recombinase/integrase	-
C1192_RS24615	2112..2240	-	129	WP_101958063.1	transcriptional regulator	-
C1192_RS24620	2867..3667	+	801	WP_038355954.1	hypothetical protein	-
C1192_RS24625	4220..4444	+	225	WP_038355953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C1192_RS24630	4441..4830	+	390	WP_038355952.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
C1192_RS24640	4998..6170	+	1173	Protein_6	IS3 family transposase	-
C1192_RS24645	6348..8645	+	2298	WP_052463081.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ipaH9.8 / ospC4	1..76160	76160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (30-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-51)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14395.54 Da
• Isoelectric Point: 7.5688

>T92628 WP_038355952.1 NZ_CP025981:4441-4830 [Escherichia marmotae]
MKLVSSQEVVAFHDRLVQAYGGLAGMADPWRAEAIIHRVLNMHHYSGVDDIYELAAIYLVAISRGHIFNDGNKRTALFTT
QVFLKRNGIHLQQNITEVLIDITLKAATGALPPHELAQRLRDVSTPQQP

Nucleotide

• Length: 390 bp

>T92628 NZ_CP025981:4441-4830 [Escherichia marmotae]
ATGAAACTTGTCAGCTCACAGGAAGTGGTAGCCTTTCACGACCGTCTGGTGCAGGCTTATGGCGGTTTGGCGGGGATGGC
AGATCCCTGGCGGGCGGAAGCGATAATTCACCGCGTCCTTAATATGCACCATTACAGCGGCGTGGATGATATCTACGAAC
TGGCCGCGATTTATCTGGTGGCAATATCTCGCGGTCATATTTTCAACGACGGCAATAAGCGCACGGCGTTATTTACCACG
CAAGTGTTTTTAAAACGCAACGGCATCCATCTGCAACAGAACATCACCGAGGTTCTGATCGACATCACGCTAAAAGCAGC
CACCGGGGCATTGCCTCCACACGAACTGGCACAGAGGCTACGTGATGTGAGCACACCACAACAGCCCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8361.38 Da
• Isoelectric Point: 4.7906

>AT92628 WP_038355953.1 NZ_CP025981:4220-4444 [Escherichia marmotae]
MFNCNYSHARGNLASLLEMAVEQGVTVEITRRKGDPAILVSKEEFERYRNAALDAEFADIISTHGEVYKELADR

Nucleotide

• Length: 225 bp

>AT92628 NZ_CP025981:4220-4444 [Escherichia marmotae]
ATGTTTAACTGCAATTACTCACATGCCAGAGGCAATCTGGCATCGTTGCTCGAAATGGCGGTGGAACAGGGCGTAACGGT
TGAGATCACCCGGCGCAAGGGCGATCCTGCGATTTTGGTCAGCAAAGAAGAGTTCGAGCGTTACCGCAATGCCGCACTCG
ATGCCGAATTTGCTGACATCATCAGCACCCACGGCGAGGTGTATAAGGAACTGGCTGACCGATGA