Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1981319..1981937 Replicon chromosome
Accession NZ_CP025979
Organism Escherichia marmotae strain HT073016

Toxin (Protein)


Gene name Hha Uniprot ID V1H8E6
Locus tag C1192_RS10335 Protein ID WP_001280991.1
Coordinates 1981719..1981937 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A7U9DNY0
Locus tag C1192_RS10330 Protein ID WP_000344801.1
Coordinates 1981319..1981714 (+) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1192_RS10320 1976397..1977590 + 1194 WP_001515899.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C1192_RS10325 1977613..1980762 + 3150 WP_001132495.1 multidrug efflux RND transporter permease subunit -
C1192_RS10330 1981319..1981714 + 396 WP_000344801.1 Hha toxicity modulator TomB Antitoxin
C1192_RS10335 1981719..1981937 + 219 WP_001280991.1 hemolysin expression modulator Hha Toxin
C1192_RS10340 1982109..1982660 + 552 WP_000102537.1 maltose O-acetyltransferase -
C1192_RS10345 1982775..1983245 + 471 WP_001515897.1 YlaC family protein -
C1192_RS10350 1983409..1984959 + 1551 WP_038354588.1 EAL domain-containing protein -
C1192_RS10355 1984996..1985349 - 354 WP_000878149.1 DUF1428 domain-containing protein -
C1192_RS10365 1985729..1986040 + 312 WP_038354581.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
C1192_RS10370 1986071..1986643 - 573 WP_000779815.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8613.97 Da        Isoelectric Point: 8.9008

>T92620 WP_001280991.1 NZ_CP025979:1981719-1981937 [Escherichia marmotae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T92620 NZ_CP025979:1981719-1981937 [Escherichia marmotae]
ATGTCCGATAAACCTTTAACAAAAACCGATTATTTGATGCGTTTGCGTCGTTGTCAGACAATTGACACGCTGGAACGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTTGCAGAAT
TGACCATGAATAAGCTTTACGACAAGATCCCTTCCTCGGTGTGGAAATTTATTCGATAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 15393.22 Da        Isoelectric Point: 4.9228

>AT92620 WP_000344801.1 NZ_CP025979:1981319-1981714 [Escherichia marmotae]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSCSNYYNHG

Download         Length: 396 bp

>AT92620 NZ_CP025979:1981319-1981714 [Escherichia marmotae]
ATGGATGAATACTCACCAAAAAGACATGATATTGCACAGCTTAAGTTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCTACCTCGGCTATCAATCTGCAACTTAATGAACTCATTGAGC
ATATTGCAACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATAGATGAATATCTG
GACGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTCCAGAAATGGCGGAAGTCAGGTAACCGACT
GTTCCGTTGTTTCGTGAATGCCACGAAAGAGAATCCTGCAAGTTTATCTTGTTCGAATTATTACAACCATGGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4ICG

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DNY0

References