Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 1452796..1453439 Replicon chromosome
Accession NZ_CP025963
Organism Klebsiella pneumoniae strain WCHKP020034

Toxin (Protein)


Gene name vagD Uniprot ID A0A4S8C081
Locus tag CEA73_RS07350 Protein ID WP_032433387.1
Coordinates 1453023..1453439 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag CEA73_RS07345 Protein ID WP_001261276.1
Coordinates 1452796..1453026 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEA73_RS07320 1447818..1448905 + 1088 Protein_1395 transcriptional regulator -
CEA73_RS07325 1448908..1451148 + 2241 WP_045326788.1 NTPase -
CEA73_RS07335 1451676..1452491 - 816 WP_032433388.1 hypothetical protein -
CEA73_RS07345 1452796..1453026 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CEA73_RS07350 1453023..1453439 + 417 WP_032433387.1 type II toxin-antitoxin system VapC family toxin Toxin
CEA73_RS07355 1453595..1454575 + 981 WP_032433385.1 hypothetical protein -
CEA73_RS07360 1454770..1456341 - 1572 WP_032433383.1 ATP-binding protein -
CEA73_RS07365 1456660..1456908 + 249 WP_032433382.1 hypothetical protein -
CEA73_RS07370 1456967..1457485 + 519 WP_045326794.1 hypothetical protein -
CEA73_RS07375 1457516..1458007 + 492 WP_032433378.1 hypothetical protein -
CEA73_RS07380 1458067..1458270 + 204 WP_032433376.1 hemolysin expression modulator Hha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1437432..1477985 40553


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14974.43 Da        Isoelectric Point: 7.8921

>T92591 WP_032433387.1 NZ_CP025963:1453023-1453439 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALHLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLTLEDWVI

Download         Length: 417 bp

>T92591 NZ_CP025963:1453023-1453439 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATTATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACCGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT92591 WP_001261276.1 NZ_CP025963:1452796-1453026 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT92591 NZ_CP025963:1452796-1453026 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S8C081

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References