Detailed information of TA system TA092586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 1490355..1490963
• Replicon: chromosome
• Accession: NZ_CP025957
• Organism: Paenibacillus polymyxa strain HY96-2

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: C1A50_RS06325
• Protein ID: WP_007429335.1
• Coordinates: 1490613..1490963 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: C1A50_RS06320
• Protein ID: WP_170970806.1
• Coordinates: 1490355..1490606 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1A50_RS06305	1486547..1487179	+	633	WP_045244041.1	LysE family transporter	-
C1A50_RS06310	1487290..1488567	+	1278	WP_103042299.1	outer membrane lipoprotein-sorting protein	-
C1A50_RS06315	1488835..1490031	+	1197	WP_103042652.1	alanine racemase	-
C1A50_RS06320	1490355..1490606	+	252	WP_170970806.1	ribbon-helix-helix protein, CopG family	Antitoxin
C1A50_RS06325	1490613..1490963	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C1A50_RS06330	1491142..1493364	+	2223	WP_185156679.1	RNA-binding transcriptional accessory protein	-
C1A50_RS06335	1493468..1493602	-	135	WP_016821163.1	cortex morphogenetic protein CmpA	-
C1A50_RS06340	1493745..1494257	+	513	WP_045244039.1	SprT family protein	-
C1A50_RS06355	1494636..1495511	-	876	WP_045244038.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T92586 WP_007429335.1 NZ_CP025957:1490613-1490963 [Paenibacillus polymyxa]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T92586 NZ_CP025957:1490613-1490963 [Paenibacillus polymyxa]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTTAGCCCTACCGTGATCGTAGCAGCCATCACAGCACAGATTCAGAAGG
CAAAGCTGCCTACACACGTGGAAATCGACGCGGCGACGCATGGCTTTGATCGTGATTCTGTTGTTCTTCTGGAGCAAATC
CGGACGATCGACAAGCAAAGGCTTACCGACAAAATCACCCATTTGGATGAAGAAACCATGCGCAAAGTGAGCGATTCGCT
ACAGATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9465.86 Da
• Isoelectric Point: 6.4671

>AT92586 WP_170970806.1 NZ_CP025957:1490355-1490606 [Paenibacillus polymyxa]
MISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEEDANSTLGRTV
SGV

Nucleotide

• Length: 252 bp

>AT92586 NZ_CP025957:1490355-1490606 [Paenibacillus polymyxa]
ATGATCAGTTTGCCAGATCATCTCTTGCAGGAAGTGGACGGGATCGTAGCTATGGAGAATTCCAACCGCAGTGAATTTAT
CAGGCAGGCAATGAAGCTTTATGTAGGTGAACGTAAAAAACGTTACATTCGTGAAGCTATGCAGCGTGGATACATGGAAA
TGGCTAAAATTAACCTGACCATGGCATCTGAAGCCTTTCACGCGGAGGAAGATGCAAACAGCACCTTGGGCCGTACAGTT
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: H6CFY1