Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 154783..155426 Replicon plasmid pKPGD4
Accession NZ_CP025952
Organism Klebsiella pneumoniae subsp. pneumoniae strain GD4

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CXZ11_RS28375 Protein ID WP_001044770.1
Coordinates 155010..155426 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CXZ11_RS28370 Protein ID WP_001261282.1
Coordinates 154783..155013 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXZ11_RS28335 150006..150785 - 780 WP_013214009.1 site-specific integrase -
CXZ11_RS28340 150969..151973 - 1005 WP_011977814.1 hypothetical protein -
CXZ11_RS28345 152003..152206 - 204 WP_011977813.1 hypothetical protein -
CXZ11_RS28350 152252..152773 - 522 WP_094960442.1 hypothetical protein -
CXZ11_RS28355 152831..153223 - 393 WP_011977811.1 hypothetical protein -
CXZ11_RS28360 153260..154204 - 945 WP_011977810.1 hypothetical protein -
CXZ11_RS29260 154365..154826 - 462 WP_014343465.1 hypothetical protein -
CXZ11_RS28370 154783..155013 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CXZ11_RS28375 155010..155426 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CXZ11_RS28380 155500..157062 + 1563 WP_004206609.1 AAA family ATPase -
CXZ11_RS28385 157047..158069 + 1023 WP_000361404.1 helicase UvrD -
CXZ11_RS28390 158325..159022 + 698 Protein_207 IS1 family transposase -
CXZ11_RS28395 159051..159323 + 273 Protein_208 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA2 / blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 - 1..170821 170821


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T92585 WP_001044770.1 NZ_CP025952:155010-155426 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T92585 NZ_CP025952:155010-155426 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT92585 WP_001261282.1 NZ_CP025952:154783-155013 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT92585 NZ_CP025952:154783-155013 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References