Detailed information of TA system TA092553

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3736668..3737362
• Replicon: chromosome
• Accession: NZ_CP025950
• Organism: Escherichia coli strain SCEC020023

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: C1A20_RS19560
• Protein ID: WP_001094398.1
• Coordinates: 3736668..3737036 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: C1A20_RS19565
• Protein ID: WP_077249034.1
• Coordinates: 3737057..3737362 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1A20_RS19540	3731841..3732797	+	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
C1A20_RS19545	3732794..3734992	+	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
C1A20_RS19550	3735002..3735958	+	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
C1A20_RS19555	3736009..3736347	+	339	Protein_3497	LysR family transcriptional regulator	-
C1A20_RS19560	3736668..3737036	-	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
C1A20_RS19565	3737057..3737362	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C1A20_RS19570	3737466..3738110	-	645	WP_000086755.1	hypothetical protein	-
C1A20_RS19575	3738129..3738350	-	222	WP_000692345.1	DUF987 domain-containing protein	-
C1A20_RS19580	3738419..3738895	-	477	WP_001424026.1	RadC family protein	-
C1A20_RS19585	3738911..3739384	-	474	WP_000855059.1	antirestriction protein	-
C1A20_RS19595	3739726..3740547	-	822	WP_001234565.1	DUF945 domain-containing protein	-
C1A20_RS19605	3740648..3740856	-	209	Protein_3505	DUF905 family protein	-
C1A20_RS19610	3740957..3741412	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagX/ecpC / yagW/ecpD / yagV/ecpE	3710179..3738110	27931

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T92553 WP_001094398.1 NZ_CP025950:c3737036-3736668 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T92553 NZ_CP025950:c3737036-3736668 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT92553 WP_077249034.1 NZ_CP025950:c3737362-3737057 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT92553 NZ_CP025950:c3737362-3737057 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4