Detailed information of TA system TA092535

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 736933..737768
• Replicon: chromosome
• Accession: NZ_CP025950
• Organism: Escherichia coli strain SCEC020023

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A763DSF8
• Locus tag: C1A20_RS05035
• Protein ID: WP_047928871.1
• Coordinates: 737391..737768 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: C1A20_RS05030
• Protein ID: WP_047928872.1
• Coordinates: 736933..737301 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1A20_RS05005	734049..734867	+	819	WP_001234702.1	DUF945 domain-containing protein	-
C1A20_RS05010	734958..735443	+	486	WP_000214316.1	antirestriction protein	-
C1A20_RS05015	735458..735934	+	477	WP_148251817.1	RadC family protein	-
C1A20_RS05020	736003..736224	+	222	WP_000692353.1	DUF987 domain-containing protein	-
C1A20_RS05025	736239..736883	+	645	WP_101975700.1	antitoxin of toxin-antitoxin stability system	-
C1A20_RS05030	736933..737301	+	369	WP_047928872.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C1A20_RS05035	737391..737768	+	378	WP_047928871.1	TA system toxin CbtA family protein	Toxin
C1A20_RS05040	737765..737914	+	150	Protein_685	hypothetical protein	-
C1A20_RS05045	737990..738187	+	198	WP_096093965.1	DUF957 domain-containing protein	-
C1A20_RS05050	738272..739117	+	846	WP_072650597.1	DUF4942 domain-containing protein	-
C1A20_RS05060	739417..739923	+	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
C1A20_RS05065	740002..741843	-	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13941.88 Da
• Isoelectric Point: 7.3222

>T92535 WP_047928871.1 NZ_CP025950:737391-737768 [Escherichia coli]
MKTLPVLPGQAAGSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTHSQLINSIDILRARRATGLMTRDNYRTVNGITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T92535 NZ_CP025950:737391-737768 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCGGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCACCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGGCATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13757.47 Da
• Isoelectric Point: 5.0907

>AT92535 WP_047928872.1 NZ_CP025950:736933-737301 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEDNQLHYLADRAGIRGRFSDADAYHLDQALPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT92535 NZ_CP025950:736933-737301 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGACTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAAGACAACCAGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAAGCCCTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A763DSF8