92526

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 311977..312629 Replicon chromosome
Accession NZ_CP025943
Organism Laceyella sacchari strain FBKL4.010

Toxin (Protein)


Gene name mazF Uniprot ID A0A7W1XUR2
Locus tag C1X05_RS01745 Protein ID WP_054095634.1
Coordinates 312279..312629 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2I7SWQ4
Locus tag C1X05_RS01740 Protein ID WP_035952874.1
Coordinates 311977..312273 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1X05_RS01710 307468..307923 - 456 WP_102991359.1 DUF2269 family protein -
C1X05_RS01715 307976..308263 - 288 WP_102991360.1 antibiotic biosynthesis monooxygenase -
C1X05_RS01720 308451..308834 + 384 WP_054095631.1 holo-ACP synthase -
C1X05_RS01725 309030..310058 + 1029 WP_054095632.1 outer membrane lipoprotein carrier protein LolA -
C1X05_RS01730 310177..310605 + 429 WP_054095633.1 hypothetical protein -
C1X05_RS01735 310610..311818 + 1209 WP_102993734.1 alanine racemase -
C1X05_RS01740 311977..312273 + 297 WP_035952874.1 ribbon-helix-helix protein, CopG family Antitoxin
C1X05_RS01745 312279..312629 + 351 WP_054095634.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
C1X05_RS01750 312834..313838 + 1005 WP_102991361.1 PP2C family protein-serine/threonine phosphatase -
C1X05_RS01755 313861..314196 + 336 WP_022738669.1 STAS domain-containing protein -
C1X05_RS01760 314193..314678 + 486 WP_022738667.1 anti-sigma B factor RsbW -
C1X05_RS01765 314644..315429 + 786 WP_102991362.1 RNA polymerase sigma factor SigB -
C1X05_RS01770 315441..315638 + 198 WP_102991363.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-50)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12940.01 Da        Isoelectric Point: 5.1224

>T92526 WP_054095634.1 NZ_CP025943:312279-312629 [Laceyella sacchari]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKAYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMNRVNEALMISLGMIDF

Download         Length: 351 bp

>T92526 NZ_CP025943:312279-312629 [Laceyella sacchari]
TTGATTGTCAAGCGTGGCGATGTCTATTTCGCAGATCTTTCCCCTGTGGTCGGATCCGAACAGGGTGGGGTTCGACCTGT
CTTAGTGATACAAAATGATATTGGGAACCGATTTAGCCCCACCGTTATCGTCGCCGCGATTACAGCCCAAATCCAAAAAG
CCAAGTTACCTACGCATGTAGAAATCGATGCCAAAGCGTACGGATTTGATCGTGACTCGGTCATTTTGCTTGAACAAATT
CGGACCATCGATAAACAGAGATTGACAGATAAGATCACCCATCTTGATGATGAGATGATGAATCGAGTCAATGAAGCGTT
GATGATTAGCTTGGGTATGATTGATTTTTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11347.24 Da        Isoelectric Point: 6.3443

>AT92526 WP_035952874.1 NZ_CP025943:311977-312273 [Laceyella sacchari]
MIGWGCFILSGTKRIMISLPTNLLQEVDGMVARDNSNRSEFIRQAMKMYLQERKKRLIREMMQRGYMEMARINLNIASEA
FVAEEEAEDTLDRLVSGV

Download         Length: 297 bp

>AT92526 NZ_CP025943:311977-312273 [Laceyella sacchari]
ATGATTGGTTGGGGGTGCTTCATTTTGTCCGGAACAAAGAGAATTATGATCAGCCTGCCAACAAATTTGCTTCAAGAAGT
AGATGGTATGGTGGCGAGGGATAATTCCAATCGCAGCGAATTTATTCGTCAGGCGATGAAAATGTATCTCCAAGAGCGGA
AAAAACGTCTCATCCGCGAAATGATGCAAAGAGGCTATATGGAAATGGCTCGCATCAATCTCAACATCGCTTCTGAAGCA
TTTGTAGCGGAAGAAGAGGCTGAAGATACTTTGGATCGATTAGTTAGCGGGGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W1XUR2

Antitoxin

Source ID Structure
AlphaFold DB A0A2I7SWQ4

References