Detailed information of TA system TA092525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3168027..3168943
• Replicon: chromosome
• Accession: NZ_CP025941
• Organism: Bacillus subtilis strain BJ3-2

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: C0W65_RS16780
• Protein ID: WP_103031932.1
• Coordinates: 3168197..3168943 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4NV07
• Locus tag: C0W65_RS16775
• Protein ID: WP_003239095.1
• Coordinates: 3168027..3168197 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0W65_RS16745	3164891..3165217	+	327	WP_103031929.1	XkdW family protein	-
C0W65_RS16750	3165214..3165378	+	165	WP_103031930.1	XkdX family protein	-
C0W65_RS16755	3165423..3166262	+	840	WP_103031931.1	phage-like element PBSX protein XepA	-
C0W65_RS16760	3166315..3166584	+	270	WP_003232655.1	hemolysin XhlA family protein	-
C0W65_RS16765	3166597..3166860	+	264	WP_003232653.1	phage holin	-
C0W65_RS16770	3166873..3167766	+	894	WP_040083358.1	N-acetylmuramoyl-L-alanine amidase	-
C0W65_RS22395	3167804..3167941	-	138	WP_136654612.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
C0W65_RS16775	3168027..3168197	-	171	WP_003239095.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
C0W65_RS16780	3168197..3168943	-	747	WP_103031932.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
C0W65_RS16785	3169053..3170054	-	1002	WP_014663667.1	inorganic phosphate transporter family protein	-
C0W65_RS16790	3170067..3170684	-	618	WP_003218470.1	DUF47 domain-containing protein	-
C0W65_RS16795	3170961..3172277	-	1317	WP_014663668.1	serine/threonine exchanger	-
C0W65_RS16800	3172665..3173615	+	951	WP_069149457.1	ring-cleaving dioxygenase	-
C0W65_RS22400	3173700..3173845	+	146	Protein_3219	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.51 Da
• Isoelectric Point: 4.4749

>T92525 WP_103031932.1 NZ_CP025941:c3168943-3168197 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTQWDRYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CQYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKMVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T92525 NZ_CP025941:c3168943-3168197 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATTGTGGCTGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACATGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCAATGGGATCGGTATCTCATTGTCGCAGTCAGCTTTGCTTTGATTGATGCTTTC
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCTGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGCATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCAATATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTGCAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAATGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6555.59 Da
• Isoelectric Point: 10.8401

>AT92525 WP_003239095.1 NZ_CP025941:c3168197-3168027 [Bacillus subtilis]
MERAFQNGCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT92525 NZ_CP025941:c3168197-3168027 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACGGATGCGAGCCCAGAGCGGCCAAACCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCACACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Antitoxin

• AlphaFold DB: G4NV07