Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2368952..2369588 Replicon chromosome
Accession NZ_CP025941
Organism Bacillus subtilis strain BJ3-2

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag C0W65_RS12465 Protein ID WP_003156187.1
Coordinates 2369238..2369588 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag C0W65_RS12460 Protein ID WP_003225183.1
Coordinates 2368952..2369233 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C0W65_RS12440 2365312..2365911 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
C0W65_RS12445 2366006..2366371 + 366 WP_038428386.1 holo-ACP synthase -
C0W65_RS12450 2366537..2367553 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
C0W65_RS12455 2367667..2368836 + 1170 WP_015252766.1 alanine racemase -
C0W65_RS12460 2368952..2369233 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
C0W65_RS12465 2369238..2369588 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
C0W65_RS12470 2369703..2370527 + 825 WP_014662853.1 RsbT co-antagonist protein RsbRA -
C0W65_RS12475 2370532..2370897 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
C0W65_RS12480 2370901..2371302 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
C0W65_RS12485 2371314..2372234 + 921 WP_103031595.1 PP2C family protein-serine/threonine phosphatase -
C0W65_RS12490 2372296..2372625 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
C0W65_RS12495 2372622..2373104 + 483 WP_003234299.1 anti-sigma B factor RsbW -
C0W65_RS12500 2373070..2373858 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
C0W65_RS12505 2373858..2374457 + 600 WP_103031596.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T92524 WP_003156187.1 NZ_CP025941:2369238-2369588 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T92524 NZ_CP025941:2369238-2369588 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT92524 WP_003225183.1 NZ_CP025941:2368952-2369233 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT92524 NZ_CP025941:2368952-2369233 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAAAGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References