Detailed information of TA system TA092496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 34359..35002
• Replicon: plasmid p103605_146
• Accession: NZ_CP025921
• Organism: Escherichia coli strain 103605

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q2VNX7
• Locus tag: CXW53_RS25610
• Protein ID: WP_001044769.1
• Coordinates: 34586..35002 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: T2HRV4
• Locus tag: CXW53_RS25605
• Protein ID: WP_001261281.1
• Coordinates: 34359..34589 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXW53_RS25560	29559..29906	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
CXW53_RS25565	29903..30283	-	381	WP_001466366.1	IS66 family insertion sequence hypothetical protein	-
CXW53_RS25570	30640..31446	-	807	WP_000099345.1	site-specific integrase	-
CXW53_RS25575	31447..31752	-	306	WP_001159861.1	type II toxin-antitoxin system toxin CcdB	-
CXW53_RS25580	31754..31972	-	219	WP_000813631.1	type II toxin-antitoxin system antitoxin CcdA	-
CXW53_RS25590	32527..33216	+	690	WP_001095858.1	helix-turn-helix domain-containing protein	-
CXW53_RS25595	33250..33867	-	618	WP_001398801.1	RES family NAD+ phosphorylase	-
CXW53_RS25605	34359..34589	+	231	WP_001261281.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CXW53_RS25610	34586..35002	+	417	WP_001044769.1	type II toxin-antitoxin system VapC family toxin	Toxin
CXW53_RS25615	35178..36296	+	1119	Protein_45	IS3 family transposase	-
CXW53_RS25620	36760..38004	+	1245	WP_000115575.1	hypothetical protein	-
CXW53_RS25625	38023..38172	+	150	WP_001399277.1	ATP-binding protein	-
CXW53_RS27385	38566..38793	-	228	WP_001731352.1	bacterial lipid A biosynthesis acyltransferase family protein	-
CXW53_RS25635	39045..39671	-	627	WP_000593827.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	cssA / csfA	1..146395	146395
	-	inside	IScluster/Tn	-	cssA	6008..36205	30197

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15183.63 Da
• Isoelectric Point: 7.7805

>T92496 WP_001044769.1 NZ_CP025921:34586-35002 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKVKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T92496 NZ_CP025921:34586-35002 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGGTTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8769.89 Da
• Isoelectric Point: 4.3775

>AT92496 WP_001261281.1 NZ_CP025921:34359-34589 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPIRPTWGSFLECEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT92496 NZ_CP025921:34359-34589 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCATCCGGCCAACCTGGGGATCATTCCTAGAGTGCG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A7ZGW5

Antitoxin

• AlphaFold DB: T2HRV4