Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1847588..1848085 Replicon chromosome
Accession NZ_CP025920
Organism Escherichia coli strain 103605

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CXW53_RS09285 Protein ID WP_001173343.1
Coordinates 1847588..1847761 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CXW53_RS09290 Protein ID WP_000398619.1
Coordinates 1847789..1848085 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXW53_RS09275 1844258..1845715 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
CXW53_RS09280 1845952..1847469 + 1518 WP_001295074.1 lysine--tRNA ligase -
CXW53_RS09285 1847588..1847761 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CXW53_RS09290 1847789..1848085 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CXW53_RS09295 1848312..1848584 - 273 WP_000405647.1 N-acetyltransferase -
CXW53_RS09300 1848596..1848724 - 129 Protein_1777 4Fe-4S mono-cluster protein YjdI -
CXW53_RS09305 1848791..1849573 - 783 WP_001317493.1 IS21-like element IS100kyp family helper ATPase IstB -
CXW53_RS09310 1849570..1850592 - 1023 WP_001356151.1 IS21 family transposase -
CXW53_RS09315 1850606..1850785 - 180 WP_126130079.1 (4Fe-4S)-binding protein -
CXW53_RS09320 1850966..1852597 + 1632 WP_001216486.1 sensor histidine kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T92474 WP_001173343.1 NZ_CP025920:c1847761-1847588 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T92474 NZ_CP025920:c1847761-1847588 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT92474 WP_000398619.1 NZ_CP025920:c1848085-1847789 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT92474 NZ_CP025920:c1848085-1847789 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References