Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 6986..7479 Replicon plasmid p120899_50
Accession NZ_CP025919
Organism Escherichia coli strain 120899

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag CXW56_RS27085 Protein ID WP_001230709.1
Coordinates 6986..7240 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag CXW56_RS27090 Protein ID WP_000151456.1
Coordinates 7237..7479 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXW56_RS27045 2032..2334 - 303 WP_001096511.1 hypothetical protein -
CXW56_RS27050 2424..3083 - 660 WP_000516232.1 peptidyl-arginine deiminase -
CXW56_RS27055 3636..3959 + 324 WP_000432873.1 hypothetical protein -
CXW56_RS27060 4313..4657 + 345 WP_001146936.1 type II toxin-antitoxin system RelE/ParE family toxin -
CXW56_RS27065 4644..4994 + 351 WP_000127482.1 addiction module antitoxin -
CXW56_RS27075 5828..6343 + 516 WP_001025393.1 J domain-containing protein -
CXW56_RS27080 6447..6989 + 543 WP_001083909.1 hypothetical protein -
CXW56_RS27085 6986..7240 + 255 WP_001230709.1 hypothetical protein Toxin
CXW56_RS27090 7237..7479 + 243 WP_000151456.1 hypothetical protein Antitoxin
CXW56_RS27100 8050..8343 + 294 WP_000673431.1 hypothetical protein -
CXW56_RS27105 8648..8935 + 288 WP_000606467.1 hypothetical protein -
CXW56_RS27110 9363..9596 + 234 WP_000093745.1 hypothetical protein -
CXW56_RS27115 9672..9974 + 303 WP_000379301.1 hypothetical protein -
CXW56_RS27120 10210..10395 + 186 Protein_14 hypothetical protein -
CXW56_RS27125 10506..11327 + 822 WP_032283064.1 DUF945 domain-containing protein -
CXW56_RS27130 11624..12271 - 648 WP_072144024.1 transglycosylase SLT domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..50268 50268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T92459 WP_001230709.1 NZ_CP025919:6986-7240 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T92459 NZ_CP025919:6986-7240 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT92459 WP_000151456.1 NZ_CP025919:7237-7479 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT92459 NZ_CP025919:7237-7479 [Escherichia coli]
ATGACTATCGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References