Detailed information of TA system TA092457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 98294..98819
• Replicon: plasmid p120899_146
• Accession: NZ_CP025917
• Organism: Escherichia coli strain 120899

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: T2HS38
• Locus tag: CXW56_RS26120
• Protein ID: WP_001159861.1
• Coordinates: 98294..98599 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: T2HUI2
• Locus tag: CXW56_RS26125
• Protein ID: WP_000813631.1
• Coordinates: 98601..98819 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXW56_RS26090	94383..95405	+	1023	WP_001356151.1	IS21 family transposase	-
CXW56_RS26095	95402..96184	+	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
CXW56_RS26105	96406..96753	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
CXW56_RS26110	96750..97130	-	381	WP_001466366.1	IS66 family insertion sequence hypothetical protein	-
CXW56_RS26115	97487..98293	-	807	WP_000099345.1	site-specific integrase	-
CXW56_RS26120	98294..98599	-	306	WP_001159861.1	type II toxin-antitoxin system toxin CcdB	Toxin
CXW56_RS26125	98601..98819	-	219	WP_000813631.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
CXW56_RS26135	99374..100063	+	690	WP_001095858.1	helix-turn-helix domain-containing protein	-
CXW56_RS26140	100097..100714	-	618	WP_001398801.1	RES family NAD+ phosphorylase	-
CXW56_RS26150	101206..101436	+	231	WP_001261281.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
CXW56_RS26155	101433..101849	+	417	WP_001044769.1	type II toxin-antitoxin system VapC family toxin	-
CXW56_RS26160	102025..103143	+	1119	Protein_129	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	csfA / cssA	1..146395	146395
	-	inside	IScluster/Tn	-	cssA	72855..103052	30197

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11680.49 Da
• Isoelectric Point: 6.4674

>T92457 WP_001159861.1 NZ_CP025917:c98599-98294 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINMMFWGI

Nucleotide

• Length: 306 bp

>T92457 NZ_CP025917:c98599-98294 [Escherichia coli]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTGCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGACTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATTGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAATGCCATTAACATGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.39 Da
• Isoelectric Point: 5.0807

>AT92457 WP_000813631.1 NZ_CP025917:c98819-98601 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMKNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT92457 NZ_CP025917:c98819-98601 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTACTCAAAGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGAAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: T2HS38

Antitoxin

• AlphaFold DB: T2HUI2