Detailed information of TA system TA092441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3742014..3742639
• Replicon: chromosome
• Accession: NZ_CP025916
• Organism: Escherichia coli strain 120899

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CXW56_RS18525
• Protein ID: WP_000911328.1
• Coordinates: 3742241..3742639 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: CXW56_RS18520
• Protein ID: WP_000450524.1
• Coordinates: 3742014..3742241 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXW56_RS18495	3737817..3738287	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
CXW56_RS18500	3738287..3738859	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
CXW56_RS18505	3739005..3739883	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CXW56_RS18510	3739900..3740934	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
CXW56_RS18515	3741147..3741860	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CXW56_RS18520	3742014..3742241	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CXW56_RS18525	3742241..3742639	+	399	WP_000911328.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CXW56_RS18530	3742786..3743649	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
CXW56_RS18535	3743664..3745679	+	2016	WP_000829355.1	tRNA cytosine(34) acetyltransferase TmcA	-
CXW56_RS18540	3745753..3746451	+	699	WP_000679823.1	esterase	-
CXW56_RS18545	3746561..3746761	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14966.32 Da
• Isoelectric Point: 8.5325

>T92441 WP_000911328.1 NZ_CP025916:3742241-3742639 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYEAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T92441 NZ_CP025916:3742241-3742639 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGAGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT92441 WP_000450524.1 NZ_CP025916:3742014-3742241 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT92441 NZ_CP025916:3742014-3742241 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33