Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 2645131..2645931 Replicon chromosome
Accession NZ_CP025916
Organism Escherichia coli strain 120899

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag CXW56_RS12990 Protein ID WP_000342449.1
Coordinates 2645404..2645931 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag CXW56_RS12985 Protein ID WP_001277108.1
Coordinates 2645131..2645397 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXW56_RS12965 2640788..2641456 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
CXW56_RS12970 2641449..2642507 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
CXW56_RS12975 2642752..2643606 + 855 WP_126134446.1 RNA polymerase sigma factor RpoH -
CXW56_RS12980 2643878..2644981 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
CXW56_RS12985 2645131..2645397 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
CXW56_RS12990 2645404..2645931 + 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
CXW56_RS12995 2645928..2646311 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
CXW56_RS13000 2646735..2647844 + 1110 WP_001352761.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
CXW56_RS13005 2647892..2648818 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
CXW56_RS13010 2648815..2650092 + 1278 WP_000803798.1 branched chain amino acid ABC transporter permease LivM -
CXW56_RS13015 2650089..2650856 + 768 WP_000082110.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T92436 WP_000342449.1 NZ_CP025916:2645404-2645931 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T92436 NZ_CP025916:2645404-2645931 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT92436 WP_001277108.1 NZ_CP025916:2645131-2645397 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT92436 NZ_CP025916:2645131-2645397 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References