Detailed information of TA system TA092400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 130413..130938
• Replicon: plasmid p204446_146
• Accession: NZ_CP025911
• Organism: Escherichia coli strain 204446

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: T2HS38
• Locus tag: CXW77_RS26170
• Protein ID: WP_001159861.1
• Coordinates: 130413..130718 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: T2HUI2
• Locus tag: CXW77_RS26175
• Protein ID: WP_000813631.1
• Coordinates: 130720..130938 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXW77_RS26140	126502..127524	+	1023	WP_001356151.1	IS21 family transposase	-
CXW77_RS26145	127521..128303	+	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
CXW77_RS26155	128525..128872	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
CXW77_RS26160	128869..129249	-	381	WP_001466366.1	IS66 family insertion sequence hypothetical protein	-
CXW77_RS26165	129606..130412	-	807	WP_000099345.1	site-specific integrase	-
CXW77_RS26170	130413..130718	-	306	WP_001159861.1	type II toxin-antitoxin system toxin CcdB	Toxin
CXW77_RS26175	130720..130938	-	219	WP_000813631.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
CXW77_RS26185	131493..132182	+	690	WP_001095858.1	helix-turn-helix domain-containing protein	-
CXW77_RS26190	132216..132833	-	618	WP_001398801.1	RES family NAD+ phosphorylase	-
CXW77_RS26200	133325..133555	+	231	WP_001261281.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
CXW77_RS26205	133552..133968	+	417	WP_001044769.1	type II toxin-antitoxin system VapC family toxin	-
CXW77_RS26210	134144..135262	+	1119	Protein_169	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	csfA / cssA	1..146395	146395
	-	inside	IScluster/Tn	-	cssA	104974..135171	30197

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11680.49 Da
• Isoelectric Point: 6.4674

>T92400 WP_001159861.1 NZ_CP025911:c130718-130413 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINMMFWGI

Nucleotide

• Length: 306 bp

>T92400 NZ_CP025911:c130718-130413 [Escherichia coli]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTGCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGACTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATTGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAATGCCATTAACATGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.39 Da
• Isoelectric Point: 5.0807

>AT92400 WP_000813631.1 NZ_CP025911:c130938-130720 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMKNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT92400 NZ_CP025911:c130938-130720 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTACTCAAAGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGAAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: T2HS38

Antitoxin

• AlphaFold DB: T2HUI2