Detailed information of TA system TA092399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 30628..31163
• Replicon: plasmid p204446_146
• Accession: NZ_CP025911
• Organism: Escherichia coli strain 204446

Toxin (Protein)

• Gene name: relE
• Uniprot ID: T2HS21
• Locus tag: CXW77_RS25440
• Protein ID: WP_000222775.1
• Coordinates: 30628..30915 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: T2HSA2
• Locus tag: CXW77_RS25445
• Protein ID: WP_001139207.1
• Coordinates: 30912..31163 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXW77_RS25385	25955..26302	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
CXW77_RS25390	26302..26979	-	678	WP_001711999.1	IS66 family insertion sequence hypothetical protein	-
CXW77_RS25400	27033..28178	+	1146	Protein_34	IS66 family transposase	-
CXW77_RS27305	28217..28465	-	249	Protein_35	hypothetical protein	-
CXW77_RS25415	28819..29421	+	603	WP_000517689.1	hypothetical protein	-
CXW77_RS27310	30073..30618	-	546	WP_000926983.1	hypothetical protein	-
CXW77_RS25440	30628..30915	-	288	WP_000222775.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CXW77_RS25445	30912..31163	-	252	WP_001139207.1	plasmid stabilization protein	Antitoxin
CXW77_RS27160	31250..31417	-	168	WP_000841011.1	hypothetical protein	-
CXW77_RS25460	32120..32977	-	858	WP_000130987.1	incFII family plasmid replication initiator RepA	-
CXW77_RS25465	32970..33044	-	75	WP_057113837.1	RepA leader peptide Tap	-
CXW77_RS25475	33289..33537	-	249	WP_000083840.1	replication regulatory protein RepA	-
CXW77_RS25480	33777..34367	-	591	WP_000766827.1	DUF2726 domain-containing protein	-
CXW77_RS25485	34405..34614	-	210	WP_001353639.1	transcriptional regulator	-
CXW77_RS25490	34660..35121	-	462	WP_001233850.1	thermonuclease family protein	-
CXW77_RS25495	35367..35579	-	213	WP_001301409.1	ANR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	csfA / cssA	1..146395	146395

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11142.17 Da
• Isoelectric Point: 10.6261

>T92399 WP_000222775.1 NZ_CP025911:c30915-30628 [Escherichia coli]
MTYTVKFRDDALREWLKLDKSVQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR

Nucleotide

• Length: 288 bp

>T92399 NZ_CP025911:c30915-30628 [Escherichia coli]
ATGACTTATACGGTTAAATTCAGGGATGATGCCCTCAGGGAATGGCTGAAACTGGATAAATCCGTTCAACAACAGTTCGC
AAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCTTCAGCAAAACTACGTGGATTAAAAGATTGCTACAAAATAA
AACTTCGCGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAGTCATGTTTACAATCTCGCCAGCGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9216.45 Da
• Isoelectric Point: 4.0927

>AT92399 WP_001139207.1 NZ_CP025911:c31163-30912 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDAMDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT92399 NZ_CP025911:c31163-30912 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCTGCTGAATTGTATGAAAGGATGC
TTGATGCTATGGATGACCAGGAGCTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTACATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: T2HS21

Antitoxin

• AlphaFold DB: A0A754EQ65