Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4876209..4876827 Replicon chromosome
Accession NZ_CP025910
Organism Escherichia coli strain 204446

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CXW77_RS24195 Protein ID WP_001291435.1
Coordinates 4876609..4876827 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CXW77_RS24190 Protein ID WP_000344800.1
Coordinates 4876209..4876583 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXW77_RS24180 4871298..4872491 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CXW77_RS24185 4872514..4875663 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
CXW77_RS24190 4876209..4876583 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CXW77_RS24195 4876609..4876827 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CXW77_RS24200 4876999..4877550 + 552 WP_000102568.1 maltose O-acetyltransferase -
CXW77_RS24205 4877666..4878136 + 471 WP_000136192.1 YlaC family protein -
CXW77_RS24210 4878300..4879850 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CXW77_RS24215 4879892..4880245 - 354 WP_000878140.1 DUF1428 domain-containing protein -
CXW77_RS24225 4880624..4880935 + 312 WP_000409911.1 MGMT family protein -
CXW77_RS24230 4880966..4881538 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T92397 WP_001291435.1 NZ_CP025910:4876609-4876827 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T92397 NZ_CP025910:4876609-4876827 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT92397 WP_000344800.1 NZ_CP025910:4876209-4876583 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT92397 NZ_CP025910:4876209-4876583 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References