Detailed information of TA system TA092379

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2603569..2604194
• Replicon: chromosome
• Accession: NZ_CP025910
• Organism: Escherichia coli strain 204446

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CXW77_RS12795
• Protein ID: WP_000911328.1
• Coordinates: 2603796..2604194 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: CXW77_RS12790
• Protein ID: WP_000450524.1
• Coordinates: 2603569..2603796 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXW77_RS12765	2599372..2599842	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
CXW77_RS12770	2599842..2600414	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
CXW77_RS12775	2600560..2601438	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CXW77_RS12780	2601455..2602489	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
CXW77_RS12785	2602702..2603415	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CXW77_RS12790	2603569..2603796	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CXW77_RS12795	2603796..2604194	+	399	WP_000911328.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CXW77_RS12800	2604341..2605204	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
CXW77_RS12805	2605219..2607234	+	2016	WP_000829355.1	tRNA cytosine(34) acetyltransferase TmcA	-
CXW77_RS12810	2607308..2608006	+	699	WP_000679823.1	esterase	-
CXW77_RS12815	2608116..2608316	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14966.32 Da
• Isoelectric Point: 8.5325

>T92379 WP_000911328.1 NZ_CP025910:2603796-2604194 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYEAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T92379 NZ_CP025910:2603796-2604194 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGAGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT92379 WP_000450524.1 NZ_CP025910:2603569-2603796 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT92379 NZ_CP025910:2603569-2603796 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33