Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2115527..2116181 Replicon chromosome
Accession NZ_CP025910
Organism Escherichia coli strain 204446

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag CXW77_RS10370 Protein ID WP_000244781.1
Coordinates 2115774..2116181 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CXW77_RS10365 Protein ID WP_000354046.1
Coordinates 2115527..2115793 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXW77_RS10340 2110696..2111439 + 744 WP_000951961.1 SDR family oxidoreductase -
CXW77_RS10345 2111496..2112929 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
CXW77_RS10350 2112974..2113285 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
CXW77_RS10355 2113449..2114108 + 660 WP_000250274.1 hemolysin III family protein -
CXW77_RS10360 2114304..2115284 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
CXW77_RS10365 2115527..2115793 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CXW77_RS10370 2115774..2116181 + 408 WP_000244781.1 protein YgfX Toxin
CXW77_RS10375 2116221..2116742 - 522 WP_001055874.1 flavodoxin FldB -
CXW77_RS10380 2116854..2117750 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CXW77_RS10385 2117775..2118485 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CXW77_RS10390 2118491..2120224 + 1734 WP_000813218.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T92377 WP_000244781.1 NZ_CP025910:2115774-2116181 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T92377 NZ_CP025910:2115774-2116181 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT92377 WP_000354046.1 NZ_CP025910:2115527-2115793 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT92377 NZ_CP025910:2115527-2115793 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References