Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 672774..673271 Replicon chromosome
Accession NZ_CP025910
Organism Escherichia coli strain 204446

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CXW77_RS03340 Protein ID WP_001173343.1
Coordinates 672774..672947 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CXW77_RS03345 Protein ID WP_000398619.1
Coordinates 672975..673271 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXW77_RS03330 669444..670901 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
CXW77_RS03335 671138..672655 + 1518 WP_001295074.1 lysine--tRNA ligase -
CXW77_RS03340 672774..672947 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CXW77_RS03345 672975..673271 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CXW77_RS03350 673498..673770 - 273 WP_000405647.1 N-acetyltransferase -
CXW77_RS03355 673782..673910 - 129 Protein_640 4Fe-4S mono-cluster protein YjdI -
CXW77_RS03360 673977..674759 - 783 WP_001317493.1 IS21-like element IS100kyp family helper ATPase IstB -
CXW77_RS03365 674756..675778 - 1023 WP_001356151.1 IS21 family transposase -
CXW77_RS03370 675792..675971 - 180 WP_126130079.1 (4Fe-4S)-binding protein -
CXW77_RS03375 676152..677783 + 1632 WP_001216486.1 sensor histidine kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T92372 WP_001173343.1 NZ_CP025910:c672947-672774 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T92372 NZ_CP025910:c672947-672774 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT92372 WP_000398619.1 NZ_CP025910:c673271-672975 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT92372 NZ_CP025910:c673271-672975 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References