Detailed information of TA system TA092366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 78677..79155
• Replicon: plasmid p300709_97
• Accession: NZ_CP025904
• Organism: Escherichia coli strain 300709

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: CXV43_RS24590
• Protein ID: WP_000969994.1
• Coordinates: 78943..79155 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E3PPA5
• Locus tag: CXV43_RS24585
• Protein ID: WP_000079921.1
• Coordinates: 78677..78946 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXV43_RS24540	74027..74653	-	627	Protein_78	hypothetical protein	-
CXV43_RS24550	75947..76078	+	132	WP_064765989.1	type I toxin-antitoxin system Hok family toxin	-
CXV43_RS24555	76362..76622	+	261	WP_000083816.1	replication regulatory protein RepA	-
CXV43_RS24565	76848..76922	+	75	WP_001365705.1	RepA leader peptide Tap	-
CXV43_RS24570	76915..77772	+	858	WP_000130956.1	incFII family plasmid replication initiator RepA	-
CXV43_RS24585	78677..78946	+	270	WP_000079921.1	hypothetical protein	Antitoxin
CXV43_RS24590	78943..79155	+	213	WP_000969994.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CXV43_RS24600	79336..79539	-	204	Protein_86	IS91 family transposase	-
CXV43_RS24605	79571..79873	+	303	Protein_87	transposase	-
CXV43_RS24610	79995..81208	+	1214	WP_164544912.1	IS3 family transposase	-
CXV43_RS24615	81250..81683	-	434	Protein_89	transposase zinc-binding domain-containing protein	-
CXV43_RS24620	81683..82048	-	366	WP_000124098.1	hypothetical protein	-
CXV43_RS24625	82137..82250	+	114	Protein_91	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	cfaD' / cfaE / cfaE / cfaC / cfaB / cfaA / etpB / etpA	1..97878	97878
	-	inside	IScluster/Tn	-	-	64822..81683	16861

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-66)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 8175.38 Da
• Isoelectric Point: 8.5821

>T92366 WP_000969994.1 NZ_CP025904:78943-79155 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVHR

Nucleotide

• Length: 213 bp

>T92366 NZ_CP025904:78943-79155 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGAATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCATCGATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10224.63 Da
• Isoelectric Point: 9.2950

>AT92366 WP_000079921.1 NZ_CP025904:78677-78946 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKNMDRNGSQLIRDFMRQTVERQHNTWFRNQVEAGRQQLKCGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT92366 NZ_CP025904:78677-78946 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAAGATGCTTTTATGGCCGCTGCAAAAAACATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGGCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTAACCAGGTTG
AGGCAGGACGTCAGCAACTCAAGTGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: E3PPA5