Detailed information of TA system TA092365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 31516..32075
• Replicon: plasmid p300709_97
• Accession: NZ_CP025904
• Organism: Escherichia coli strain 300709

Toxin (Protein)

• Gene name: relE
• Uniprot ID: E3PPG0
• Locus tag: CXV43_RS24265
• Protein ID: WP_000421265.1
• Coordinates: 31800..32075 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E3PPG1
• Locus tag: CXV43_RS24260
• Protein ID: WP_001743519.1
• Coordinates: 31516..31800 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXV43_RS24245	27702..28268	+	567	WP_000904908.1	recombinase family protein	-
CXV43_RS24250	28481..28678	+	198	WP_001381161.1	hypothetical protein	-
CXV43_RS24255	29010..30762	+	1753	Protein_28	AAA family ATPase	-
CXV43_RS24260	31516..31800	+	285	WP_001743519.1	ribbon-helix-helix domain-containing protein	Antitoxin
CXV43_RS24265	31800..32075	+	276	WP_000421265.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CXV43_RS24270	32238..33314	+	1077	WP_000610004.1	hypothetical protein	-
CXV43_RS24280	33565..33846	+	282	WP_001105061.1	hypothetical protein	-
CXV43_RS25375	34065..34379	+	315	Protein_33	hypothetical protein	-
CXV43_RS24285	34377..34451	-	75	Protein_34	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
CXV43_RS24295	35865..36260	-	396	WP_000445942.1	plasmid partitioning/stability family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	cfaD' / cfaE / cfaE / cfaC / cfaB / cfaA / etpB / etpA	1..97878	97878
	-	flank	IS/Tn	-	-	24523..27657	3134

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10742.45 Da
• Isoelectric Point: 9.9324

>T92365 WP_000421265.1 NZ_CP025904:31800-32075 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQASVILLTHPRMGEQLFQFELREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T92365 NZ_CP025904:31800-32075 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCATCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACTCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGTCTGTGGCATACGCGGGAAAACAGATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10490.95 Da
• Isoelectric Point: 5.6546

>AT92365 WP_001743519.1 NZ_CP025904:31516-31800 [Escherichia coli]
MQMKNNCAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQIIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT92365 NZ_CP025904:31516-31800 [Escherichia coli]
ATGCAAATGAAAAACAATTGTGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAGCAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTAACATCCGGACAGATTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: E3PPG0

Antitoxin

• AlphaFold DB: E3PPG1